Recombinant Cynomolgus Monkey B7-1/CD80 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-1/CD80 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) that produces 50% of the optimal binding response is approximately 5-30 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Val35-Asn242, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Val35
  • Predicted Molecular Mass
    25 kDa
  • SDS-PAGE
    35-45 kDa, reducing conditions
9244-B1
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Cynomolgus Monkey
B7-1/CD80 (Catalog # 9244-B1) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) binds with an ED50 of 5-30 ng/mL.
Background: B7-1/CD80
B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature cynomolgus B7-1 consists of a 208 aa extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, cynomolgus B7-1 shares 97%, 51%, and 54% aa sequence identity with human, mouse, and rat B7-1, respectively. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).
  • References:
    1. Ville, S. et al. (2015) Front. Immunol. 6:411.
    2. Azuma, M. et al. (1993) Nature 366:76.
    3. Freeman, G.J. et al. (1993) Science 262:909.
    4. Chen, C. et al. (1994) J. Immunol. 152:4929.
    5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
    6. Lanier, L. et al. (1995) J. Immunol. 154:97.
    7. Freeman, G.J. et al. (1989) J. Immunol. 143:2714.
  • Entrez Gene IDs:
    941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
  • Alternate Names:
    Activation B7-1 antigen; B7; B71; B7-1/CD80; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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