Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of biotinylated recombinant human GITR Ligand/TNFSF18 that produces 50% of the optimal binding response is 8-40 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived
    Cynomolgus Monkey GITR/TNFRSF18
    (Gln20-Glu155)
    Accession # XP_005545180
    IEGRMDHuman IgG1
    (Pro100-Lys330)
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln20 inferred from enzymatic pyroglutamate treatment revealing Arg21
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    41 kDa
  • SDS-PAGE
    49-60 kDa, reducing conditions
9428-GR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Cynomolgus Monkey GITR/TNFRSF18 Fc Chimera (Catalog # 9428‑GR) is immobilized at 0.5 µg/mL, biotinylated recombinant human GITRLigand/TNFSF18 binds with an ED50 of 8-40 ng/mL.
Background: GITR/TNFRSF18
GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature human GITR consists of a 137 amino acid (aa) extracellular domain (ECD) with three tandem TNF R cysteine-rich repeats, a 21 aa transmembrane segment, and a 58 aa cytoplasmic domain (3, 4). Within the ECD region, cyno GITR shares 89.8% and 53.2% aa sequence identity with human and mouse GITR, respectively. Alternative splicing generates an isoform with a short deletion in the cytoplasmic domain and a potentially secreted isoform that is substituted within the third TNF R repeat and lacks the transmembrane and cytoplasmic regions. GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (4-6), and it is up-regulated on antigen-activated conventional CD4+ and CD8+ T cells (3, 4, 6, 7). GITR binding by GITR Ligand/TNFSF18 co-stimulates the proliferation and activation of CD4+ or CD8+ conventional T cells (3, 7-9). It also induces the proliferation of Treg (8, 10) but inhibits the ability of Treg to suppress immune responses (5, 8, 11-13). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 11), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (10, 14). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (15).
  • References:
    1. Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
    2. Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
    3. Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
    4. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
    5. Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
    6. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
    7. Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
    8. Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
    9. Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
    10. Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
    11. Ko, K. et al. (2005) J. Exp. Med. 202:885.
    12. Ji, H. et al. (2004) J. Immunol. 172:5823.
    13. Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
    14. Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
    15. O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.
  • Long Name:
    Glucocorticoid Induced TNF Receptor Family Related Gene
  • Entrez Gene IDs:
    8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)
  • Alternate Names:
    Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18
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