Recombinant Cynomolgus/Rhesus Macaque B7-2/CD86 Protein, CF

Name: Recombinant Cynomolgus/Rhesus Macaque B7-2/CD86 Protein, CF
Brand: R&D Systems
SKU: 9798-B2
Price: 454 USD
Availability: InStock

R&D Systems | Catalog # 9798-B2

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Fc Chimera

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems HEK293-derived Recombinant Cynomolgus/Rhesus Macaque B7-2/CD86 Protein (9798-B2)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

XP_005548057

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity

View all B7-2/CD86 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived B7-2/CD86 protein

Cynomolgus Monkey/Rhesus Macque B7-2 (Leu20-His239) Accession # XP_005548057	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

52 kDa

SDS-PAGE

73-100 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera (Catalog # 9336-CT) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque B7‑2/CD86 Fc Chimera (Catalog# 9798-B2) binds with an ED₅₀ of 0.2-1 μg/mL.

Scientific Data Images for Recombinant Cynomolgus/Rhesus Macaque B7-2/CD86 Protein, CF

Recombinant Cynomolgus/Rhesus Macaque B7-2/CD86 Protein Binding Activity

When Recombinant Cynomolgus Monkey CTLA-4 Fc Chimera (Catalog # 9336-CT) is immobilized at 0.5 µg/mL, Recombinant Cynomolgus Monkey/Rhesus Macaque B7-2/CD86 Fc Chimera (Catalog # 9798-B2) binds with an ED₅₀ of 0.2-1 µg/mL.

Formulation, Preparation, and Storage

9798-B2

Formulation	Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water. Loading...
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: B7-2/CD86

B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Within the ECD, cynomolgus B7-2 shares 96%, 57%, and 56% amino acid sequence identity with human, mouse and rat B7-2, respectively. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (2-5), as well as on vascular endothelial cells (6). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co-stimulation (3, 5, 7-9). B7-1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up‑regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (3, 5, 7, 8). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (10). B7-2 is expressed earlier than B7-1 following APC activation (4), and both proteins bind with higher affinity to CTLA-4 than to CD28 (8). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (11). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (12). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (13).