Recombinant Human 19S Proteasome Protein, CF

Newer Version Available: E-367
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Discontinued Product

E-366 has been discontinued and is replaced by E-367.

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Citations (6)
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Recombinant Human 19S Proteasome Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Bioassay data are not available.
Source
Human embryonic kidney cell, HEK293-derived human 19S Proteasome protein
Predicted Molecular Mass
700 kDa

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E-366

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-366

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 7.5, 20 mM NaCl, 15% Glycerol, 1 mM DTT

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: 19S Proteasome

The 19S Proteasome, also known as PA700, is a multi-subunit regulatory particle that associates with the 20S Proteasome core particle to form the 26S Proteasome. The 19S Proteasome can cap the 20S Proteasome at one or both ends and regulate substrate access to proteolytic activity in an ATP-dependent manner by modulating the conformation of the 20S Proteasome and facilitating substrate access to the 20S Proteasome catalytic core (1-3). The 19S Proteasome recognizes ubiquitinated proteins as well as non-ubiquitinated, misfolded proteins. It catalyzes substrate deubiquination, unfolds substrate proteins, and threads them into the 20S Proteasome catalytic core (4,5). The 19S Proteasome consists of a base subcomplex and a lid subcomplex. The base subcomplex is composed of six AAA+ family members, two scaffolding proteins, and regulatory proteins involved in Ubiquitin recognition (6,7). The 19S Proteasome lid subcomplex contains eight subunits and one deubiquitinating enzyme, Rpn11 (6,7). Although inhibitors targeting the 19S Proteasome regulatory particle have been developed, they are under-utilized in comparison with proteasome inhibitors targeting the 20S Proteasome core particle (8).

This highly purified 19S Proteasome preparation (from the transformed HEK cell line) can be used in vitro for the activation of the 20S Proteasome, as well as for deubiquitination of target substrates via Rpn11. 

References
  1. Adams, G.M. et al. (1998) Biochemistry 37:12927.
  2. Chu-Ping, M. et al. (1994) J. Biol. Chem. 269:3539.
  3. Kohler, A. et al. (2001) Mol. Cell 7:1143.
  4. Strickland, E. et al. (2000) J. Biol. Chem. 275:5565.
  5. Finley, D. (2009) Annu. Rev. Biochem. 78:477.
  6. Stadtmueller, B.M. & C.P. Hill (2011) Mol. Cell 41:8.
  7. Xie, Y. (2010) J. Mol. Cell Biol. 2:308.
  8. Kisselev, A.F. et al. (2012) Chem. Biol. 19:99.
Alternate Names
19S Proteasome; PA700

Citations for Recombinant Human 19S Proteasome Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Cytotoxic unsaturated electrophilic compounds commonly target the ubiquitin proteasome system
    Authors: K Selvaraju, A Mofers, P Pellegrini, J Salomonsso, A Ahlner, V Morad, EK Hillert, B Espinosa, ESJ Arnér, L Jensen, J Malmström, MV Turkina, P D'Arcy, MA Walters, M Sunnerhage, S Linder
    Sci Rep, 2019-07-08;9(1):9841.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Radix Tetrastigma Hemsleyani Flavone Suppresses Cutaneous Squamous Cell Carcinoma A431 Cells via Proteasome Inhibition
    Authors: L Zhong, X Yang, Y Zhu, J Peng, Y Cao
    Med. Sci. Monit., 2019-01-15;25(0):436-442.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. TRIM11 activates the proteasome and promotes overall protein degradation by regulating USP14
    Authors: L Chen, G Zhu, EM Johns, X Yang
    Nat Commun, 2018-03-26;9(1):1223.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  4. 15-Deoxy-?(12,14)-Prostaglandin J2 Modifies Components of the Proteasome and Inhibits Inflammatory Responses in Human Endothelial Cells
    Authors: Desmond J Fitzgerald
    Front Immunol, 2016-10-27;7(0):459.
    Applications: Bioassay
  5. Small-molecule RA-9 inhibits proteasome-associated DUBs and ovarian cancer in vitro and in vivo via exacerbating unfolded protein responses.
    Authors: Coughlin K, Anchoori R, Iizuka Y, Meints J, MacNeill L, Vogel R, Orlowski R, Lee M, Roden R, Bazzaro M
    Clin Cancer Res, 2014-04-11;20(12):3174-86.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  6. A bis-benzylidine piperidone targeting proteasome ubiquitin receptor RPN13/ADRM1 as a therapy for cancer.
    Authors: Anchoori R, Karanam B, Peng S, Wang J, Jiang R, Tanno T, Orlowski R, Matsui W, Zhao M, Rudek M, Hung C, Chen X, Walters K, Roden R
    Cancer Cell, 2013-12-09;24(6):791-805.
    Applications: Bioassay

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