Recombinant Human ADAM22 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Supplemental Products

Recombinant Human ADAM22 Protein, CF Summary

Product Specifications

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED50 for this effect is 20-100 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human ADAM22 protein
Gly26-His528 (Leu78Val), with a C-terminal 8-His tag
Accession #
N-terminal Sequence
Gly26 & Asn233
Structure / Form
Mixture of pro and mature forms
Predicted Molecular Mass
58 kDa (pro) & 34 kDa (mature)
40-45 kDa & 69-80 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: ADAM22

A disintegrin and metalloproteinase 22 (ADAM22), also known as MDC2, is an 85-90 kDa transmembrane member of the M12B family of peptidases (1). Human ADAM22 contains an N-terminal 197 amino acid (aa) cleavable proregion and a 514 aa extracellular domain (ECD). The ECD contains a nonfunctional metalloprotease domain, an integrin‑binding disintegrin domain, a cysteine‑rich region, and one EGF-like domain (2). The cytoplasmic portion contains two phosphorylation motifs which bind to 14-3-3 proteins to mediate cell adhesion (3, 4). Alternative splicing generates multiple isoforms with a variety of insertions, deletions, and substitutions in the cytoplasmic region (2, 5, 6). Within the peptidase-like and disintegrin domains, human ADAM22 shares 96% sequence identity with mouse and rat ADAM22. ADAM22 is expressed on neurons, astrocytes, and Schwann cells in the cerebral cortex, hippocampus, and cerebellum and also on some low-grade gliomas (5, 7-9). It can be up-regulated in endocrine therapy-resistant breast cancer, and it contributes to tumor cell migration (10). It mediates cell adhesion through binding to Integrins alpha 6, alpha 9, and beta 3 (7). ADAM22 is localized to post-synaptic densities (11). It binds the secreted protein LGI1 which functions as a bridge to link ADAM22 with pre-synaptically expressed ADAM23 (9). The interaction of LGI1 with ADAM22 promotes AMPA receptor expression in hippocampal neurons and strengthens excitatory synapses (9). In axonal juxtaparanodes, ADAM22 is a component of the voltage-gated Kv1 potassium channel that responds to LGI1 (12). It also colocalizes with Kv1 channels in basket cell interneuron terminals of the cerebellum (12). ADAM22 additionally binds to Schwann cell derived LGI4 and is required for LGI4 to promote axon myelination (8). Mice lacking ADAM22 expression exhibit hypomyelination and profound ataxia (13).

  1. Edwards, D.R. et al. (2008) Mol. Aspects Med. 29:258.
  2. Sagane, K. et al. (1998) Biochem. J. 334:93.
  3. Godde, N.J. et al. (2006) J. Cell Sci. 119:3296.
  4. Zhu, P. et al. (2005) Biochem. Biophys. Res. Commun. 331:938.
  5. Harada, T. et al. (2000) Jpn. J. Cancer Res. 91:1001.
  6. Godde, N.J. et al. (2007) Gene 403:80.
  7. D’Abaco, G.M. et al. (2006) Neurosurgery 58:179.
  8. Ozkaynak, E. et al. (2010) J. Neurosci. 30:3857.
  9. Fukata, Y. et al. (2006) Science 313:1792.
  10. McCartan, D. et al. (2012) Cancer Res. 72:220.
  11. Fukata, Y. et al. (2010) Proc. Natl. Acad. Sci. USA 107:3799.
  12. Ogawa, Y. et al. (2010) J. Neurosci. 30:1038.
  13. Sagane, K. et al. (2005) BMC Neurosci. 6:33.
Long Name
A Disintegrin and Metalloproteinase Domain 22
Entrez Gene IDs
53616 (Human); 11496 (Mouse); 57033 (Rat)
Alternate Names
ADAM 22; ADAM metallopeptidase domain 22; ADAM22; MDC2; metalloproteinase-disintegrin ADAM22-3; metalloproteinase-like, disintegrin-like, and cysteine-rich protein 2; MGC149832


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human ADAM22 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human ADAM22 Protein, CF and earn rewards!

Have you used Recombinant Human ADAM22 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review