Recombinant Human ADAM9 Protein, CF

R&D Systems | Catalog # 939-AD

R&D Systems
Discontinued Product
939-AD has been discontinued. View all ADAM9 products.

Key Product Details

Source

NS0

Accession Number

Q13443

Structure / Form

Mature form

Applications

Enzyme Activity
View all ADAM9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ADAM9 protein
Ala206-Asp697, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala206

Predicted Molecular Mass

55 kDa

SDS-PAGE

64 kDa, reducing conditions

Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (Catalog # ES003).
The specific activity is >1.0 pmol/min/µg, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 5 using 939-AD in the following applications:

Formulation, Preparation, and Storage

939-AD
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ADAM9

ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease‑like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc‑binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75 TNF receptor, the beta -amyloid protein precursor, and the c‑kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane‑anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta -chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3
(SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

References

  1. Moss, et al. (2001) DDT 6:417.
  2. Roghan, et al. (1999) J. Biol. Chem. 274:3531.
  3. Izumi, et al. (1998) EMBO J. 17:7260.
  4. Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
  5. Nath, et al. (2000) J. Cell Sci. 113:2319.
  6. Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
  7. Howard, et al. (1999) J. Biol. Chem. 274:31693.
  8. Weskamp, et al. (1996) J. Cell. Biol. 132:717.

Long Name

A Disintegrin and Metalloprotease-like Domain 9

Alternate Names

MCMP, MDC9, Meltrin gamma

Entrez Gene IDs

8754 (Human); 11502 (Mouse)

Gene Symbol

ADAM9

UniProt

Q13443

Additional ADAM9 Products

Product Documents for Recombinant Human ADAM9 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human ADAM9 Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Recombinant Human ADAM9 Protein, CF

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Protocols

View specific protocols for Recombinant Human ADAM9 Protein, CF (939-AD):

Materials
  • Assay Buffer: 25 mM Tris, 2.5 µM ZnCl2, 0.005% (w/v) Brij-35, pH 9.0
  • Recombinant Human ADAM-9 (rhADAM9) (Catalog # 939-AD)
  • Fluorogenic Peptide Substrate III: MCA-Pro-Leu-Ala-Gln-Ala-Val-DPA-Arg-Ser-Ser-Ser-Arg-NH2 (Catalog # ES003)
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhADAM9 to 40 µg/mL in Assay Buffer.
  2. Dilute substrate to 20 µM in Assay Buffer.
  3. Combine equal volumes of 40 µg/mL rhADAM9 and 20 µM substrate. Include a control containing Assay Buffer and 20 µM substrate without any rhADAM9.
  4. Incubate reactions at 37 °C for 30 minutes.
  5. Load 100 μL of reactions and control per well in a black plate.
  6. Read with excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively, in endpoint mode.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Fluorescence* (RFU) x Conversion Factor** (pmol/RFU)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Control
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:

  • rhADAM9: 2.0 µg
  • Substrate: 10 µM

FAQs

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