Recombinant Human ADAMTS15 Protein, CF

R&D Systems | Catalog # 5149-AD

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human ADAMTS15 Protein (5149-AD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q8TE58

Applications

Enzyme Activity
View all ADAMTS15 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ADAMTS15 protein
Gly18-Cys682 (pro) & Phe213-Cys682 (mature), both with a C-terminal 10-His tag

Purity

>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly18 & Phe213

Predicted Molecular Mass

74 kDa (pro), 53 kDa (mature)

SDS-PAGE

85 kDa and 65 kDa, reducing conditions

Activity

Measured by its ability to cleave Recombinant Human Aggrecan G1-IGD-G2 Domains (Catalog # 1220-PG).
Cleavage of 1 µg of rhAggrecan is >90%, as measured under the described conditions.

Reviewed Applications

Read 1 review rated 4 using 5149-AD in the following applications:

Formulation, Preparation, and Storage

5149-AD
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ADAMTS15

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) is a group of nineteen secreted zinc binding metalloproteases that is involved in development, inflammation, angiogenesis and several pathological conditions (1, 2). They are synthesized as zymogens which have a pro-domain that is removed by furin-like protein convertases. The mature secreted enzymes consist of catalytic, disintegrin-like, type I thrombospondin (TSP) motif, cysteine-rich, and spacer domains followed by zero to fourteen additional TSP motifs. Among ADAMTS proteases, ADAMTS1, -4, -5, -8, and -15 form a subfamily that possess aggrecanase activity (1-3). While ADAMTS4 and -5 (also known as aggrecanse-1 and -2, respectively) (4) have been well studied, the enzymatic activity of ADAMTS15 remains to be explored. It is suggested that ADAMTS15 may be involved in aggrecan degradation and breast cancer (5, 6). ADAMTS15 may also exhibit tumor suppressor activities since it contains genetic mutations in human colorectal carcinomas and restrains tumor growth and invasion in both in vitro and in vivo studies (7). The purified rhADAMTS15 is truncated and ends before the spacer domain.

References

  1. Porter, S. et al. (2005) Biochem. J. 386:15.
  2. Jones, G.C. and G.P. Riley (2005) Arthritis Res. Ther. 7:160.
  3. Nicholson, A.C. et al. (2005) BMC Evol. Biol. 5:11.
  4. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  5. Porter, S. et al. (2006) Intl. J. Cancer 118:1241.
  6. Kevorkian, L. et al. (2004) Arthritis Rheum. 50:131.
  7. Viloria, C.G. et al. (2009) Cancer Res. 69:4926.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 15

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 15, preproprotein, ADAM metallopeptidase with thrombospondin type 1 motif, 15, ADAM-TS 15, ADAM-TS15, ADAMTS-15, EC 3.4.24, EC 3.4.24.-, EC 3.4.24.14, EC 3.4.24.82, metalloprotease disintegrin 15 with thrombospondin domains, MGC126403

Entrez Gene IDs

170689 (Human); 235130 (Mouse); 300474 (Rat)

Gene Symbol

ADAMTS15

UniProt

Q8TE58

Additional ADAMTS15 Products

Product Documents for Recombinant Human ADAMTS15 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human ADAMTS15 Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Recombinant Human ADAMTS15 Protein, CF

Customer Reviews for Recombinant Human ADAMTS15 Protein, CF (1)

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  • Recombinant Human ADAMTS15 Protein, CF
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 09/23/2019
    I used this protein as a standard for developing an ELISA in combination with pAb (cat. AF5149) and mAb (cat. MAB 5149). This combination works well for a sandwich ELISA detection human ADAMTS 14 in serum.
    Recombinant Human ADAMTS15 Protein, CF 5149-AD

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Protocols

View specific protocols for Recombinant Human ADAMTS15 Protein, CF (5149-AD):

Materials
  • Dilution Buffer: 50 mM Tris, 0.02% (w/v) Brij-35, pH 7.5
  • Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5 (TCNB)
  • Recombinant Human Aggrecan G1-IGD-G2 Domains (rhAggrecan) (Catalog # 1220-PG)
  • Recombinant Human ADAMTS15 (rhADAMTS15) (Catalog # 5149-AD)
  • SDS-PAGE and/or Western blot
  1. Dilute rhADAMTS15 to 25 μg/mL in Dilution Buffer.
  2. Incubate on ice for 4 hours.
  3. Dilute rhADAMTS15 to 20 µg/mL in Assay Buffer.
  4. Dilute rhAggrecan to 250 µg/mL in Assay Buffer.
  5. Combine equal amounts diluted rhAggrecan and diluted rhADAMTS15. As controls, combine equal amounts Aggrecan and Assay Buffer as well as equal amounts rhADAMTS15 and Assay Buffer.
  6. Incubate the reaction mixture and controls overnight at 37 °C.
  7. Combine 30 µL of reactions (and controls) with 26.2 µL reducing SDS-PAGE sample buffer.
  8. Analyze the cleavage by SDS-PAGE (load 15 µL per lane) followed by protein staining and/or Western blot.

Per Lane:

  • rhADAMTS15: 0.080 µg
  • rhAggrecan: 1 µg

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