Recombinant Human Angiopoietin-like 5 Protein, CF

R&D Systems | Catalog # 6675-AN/CF

R&D Systems
100% Guarantee

Key Product Details

Source

CHO

Accession Number

AAH49170

Applications

Bioactivity
View all Angiopoietin-like Protein 5/ANGPTL5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 5/ANGPTL5 protein
Met1-Lys388 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23 & Asn26

Predicted Molecular Mass

42.3 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 80.0-800 ng/mL.

Scientific Data Images for Recombinant Human Angiopoietin-like 5 Protein, CF

Recombinant Human Angiopoietin‑like Protein 5/ANGPTL5 Binding Activity.

When Recombinant Human Angiopoietin-like Protein 5/ANGPTL5 (Catalog # 6675-AN/CF) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera (2078-T4) binds with an ED50 of 80.0-800 ng/mL.

Formulation, Preparation, and Storage

6675-AN/CF
Formulation Supplied as a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Angiopoietin-like Protein 5/ANGPTL5

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled‑coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).

References

  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Zeng, L. et al. (2003) J. Hum. Genet. 48:159.
  4. Romeo, S. et al. (2009) J. Clin. Invest. 119:70.
  5. Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156.
  6. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
  7. Zhang, C.C. et al. (2008) Blood 111:3415.
  8. Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.

Alternate Names

Angiopoietin like Protein 5, ANGPTL5

Entrez Gene IDs

253935 (Human)

Gene Symbol

ANGPTL5

UniProt

AAH49170

Additional Angiopoietin-like Protein 5/ANGPTL5 Products

Product Documents for Recombinant Human Angiopoietin-like 5 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Angiopoietin-like 5 Protein, CF

For research use only

Customer Reviews for Recombinant Human Angiopoietin-like 5 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human Angiopoietin-like 5 Protein, CF and earn rewards!

Have you used Recombinant Human Angiopoietin-like 5 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Recombinant Human Angiopoietin-like 5 Protein, CF

Showing  1 - 1 of 1 FAQ Showing All

  • Q:

    This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Proteins and Enzymes