Recombinant Human Angiopoietin-like 5 Protein

R&D Systems | Catalog # 6675-AN

R&D Systems
Discontinued Product
6675-AN has been discontinued. An alternative/replacement product is available: 6675-AN/CF. View all Angiopoietin-like Protein 5/ANGPTL5 products.

Key Product Details

Source

CHO

Accession Number

AAH49170

Applications

Bioactivity
View all Angiopoietin-like Protein 5/ANGPTL5 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 5/ANGPTL5 protein
Met1-Lys388 with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23 & Asn26

Predicted Molecular Mass

42.3 kDa

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL).
The ED50 for this effect is 70-280 ng/mL in the presence of a cross‑linking Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).

Reviewed Applications

Read 1 review rated 5 using 6675-AN in the following applications:

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 6675-AN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 6675-AN/CF
Formulation Supplied as a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 5/ANGPTL5

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled‑coil domain and a C-terminal fibrinogen-like domain (1-3). It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue (3, 4). Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index (4, 5). ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells (6-8). The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear (6).

References

  1. Oike, Y. et al. (2009) Circ. J. 73:2192.
  2. Miida, T. and S. Hirayama (2010) Curr. Opin. Lipidol. 21:70.
  3. Zeng, L. et al. (2003) J. Hum. Genet. 48:159.
  4. Romeo, S. et al. (2009) J. Clin. Invest. 119:70.
  5. Liu, D.J. and S.M. Leal (2010) PloS Genet. 6:e1001156.
  6. Zhang, C.C. et al. (2006) Nat. Med. 12:240.
  7. Zhang, C.C. et al. (2008) Blood 111:3415.
  8. Khoury, M. et al. (2010) Stem Cells Dev. Dec 8 epub.

Alternate Names

Angiopoietin like Protein 5, ANGPTL5

Entrez Gene IDs

253935 (Human)

Gene Symbol

ANGPTL5

UniProt

AAH49170

Additional Angiopoietin-like Protein 5/ANGPTL5 Products

Product Documents for Recombinant Human Angiopoietin-like 5 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 6675-AN (with carrier)
Download SDS 6675-AN/CF (carrier free)

Product Specific Notices for Recombinant Human Angiopoietin-like 5 Protein

For research use only

Customer Reviews for Recombinant Human Angiopoietin-like 5 Protein (1)

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FAQs for Recombinant Human Angiopoietin-like 5 Protein

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  • Q:

    This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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