Recombinant Human Angiopoietin-like 6 Protein

Formulations:
Catalog # Availability Size / Price Qty
8466-AN-025
ANGPTL6 Promotes Rat Liver Mononuclear Cell Expansion.
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Recombinant Human Angiopoietin-like 6 Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 8-40 ng/mL in the presence of a cross-linking Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # MAB3696).
Source
Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 6/ANGPTL6 protein
Ala20-Leu470, with an N-terminal c-Myc tag
Accession #
N-terminal Sequence
Analysis
Glu (c-Myc tag)
Structure / Form
Disulfide-linked homo-oligomer
Predicted Molecular Mass
51 kDa
SDS-PAGE
58-69 kDa and 31-41 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8466-AN

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

8466-AN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity ANGPTL6 Promotes Rat Liver Mononuclear Cell Expansion. View Larger

Recombinant Human ANGPTL6 promotes the expansion of E16 rat liver mononuclear cells in the presence of Recombinant Mouse SCF/c-kit, Recombinant Mouse Thrombopoietin/TPO, and Recombinant Mouse Flt-3 Ligand. The ED50for this effect is 8-40 ng/ml in the presence of a cross-linking Mouse Anti-Human c-Myc Monoclonal Antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 6/ANGPTL6

Angiopoietin-­like Protein 6 (ANGPTL6), also known as angiopoietin-­related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled­-coil domain and a fibrinogen-­like domain (1, 2). Mature human ANGPTL6 shares 75% sequence identity with both mouse and rat ANGPTL6. ANGPTL6 is highly expressed in hepatocytes and is detectable in serum (1, 2). Within the liver, secreted ANGPTL6 inhibits gluconeogenesis and promotes insulin sensitivity and energy expenditure (3, 4). Circulating ANGPTL6 promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis (5, 6). An integrin­-binding RGD motif located within the fibrinogen-­like domain of ANGPTL6, enables it to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells (5, 7). Elevated levels of ANGPTL6 in serum are associated with metabolic syndrome, diabetes, and pre-eclampsia (8-11). Serum ANGPTL6 levels are also elevated during obesity (4, 12). ANGPTL6 is reported to promote the survival and expansion of hematopoietic stem cells (13). ANGPTL6 is produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells (7).

References
  1. Oike, Y. and M. Tabata (2009) Circ. J. 73:2192.
  2. Kadomatsu, T. et al. (2011) FEBS J. 278:559.
  3. Kitazawa, M. et al. (2007) J. Pharmacol. Exp. Ther. 323:787.
  4. Oike, Y. et al. (2005) Nat. Med. 11:400.
  5. Oike, Y. et al. (2003) Proc. Natl. Acad. Sci. U S A 100:9494.
  6. Okazaki, H. et al. (2012) J. Dermatol. 39:366.
  7. Zhang, Y. et al. (2006) Biochem. Biophys. Res. Commun. 347:100.
  8. Legry, V. et al. (2009) Diabetes. Metab. 35:287.
  9. Namkung, J. et al. (2011) Metabolism 60:564.
  10. Stepan, H. et al. (2009) Am. J. Hypertens. 22:314.
  11. Ebert, T. et al. (2009) Metabolism 58:547.
  12. Ebert, T. et al. (2014) Horm. Metab. Res. 46:685.
  13. Akhter, S. et al. (2013) Protein Cell 4:220.
Entrez Gene IDs
83854 (Human); 70726 (Mouse); 298698 (Rat)
Alternate Names
AGF; AGFAngiopoietin-related protein 5; Angiopoietin like Protein 6; angiopoietin-like 6; Angiopoietin-like Protein 6; Angiopoietin-related growth factor; ANGPTL6; ARP5; ARP5angiopoietin-related protein 6

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