Recombinant Human Apolipoprotein M Protein, CF Summary
Cys23-Asn188, with an N-terminal Met and 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Apolipoprotein M/ApoM
Apolipoprotein M (ApoM) is an approximately 25 kDa variably glycosylated protein that adopts a beta-barrel structure characteristic of lipocalin family proteins. It functions as a component of lipoprotein particles which play essential roles in fatty acid and cholesterol transport and metabolism (1, 2). Human ApoM shares 83% amino acid sequence identity with mouse and rat ApoM (3). Alternative splicing generates a short isoform that lacks the N-terminal 72 amino acids. ApoM is produced primarily by hepatocytes but also by renal tubule epithelial cells (4). The signal peptide is not cleaved and is required for ApoM association with lipoprotein particles as well as Megalin mediated reabsorption by the kidney (5, 6). ApoM is cleared from the circulation by the ubiquitously expressed LDL R (7, 8). The majority of circulating ApoM is associated with HDL, while a smaller fraction is associated with LDL (3, 9, 10). It promotes the intracellular formation of large pre-beta (discoidal) HDL particles which inhibit the development of atherosclerotic plaques by facilitating the efflux of cellular cholesterol and preventing phospholipid oxidation (7, 9, 11‑13). The lipocalin structure of ApoM enables it to function as a carrier protein for small hydrophobic molecules including retinol (Vitamin A), all-trans-retinoic acid, 9-cis-retinoic acid, and sphingosine-1-phosphate (S1P) (14, 15). ApoM delivers S1P to S1P1 receptors and contributes to the maintenance of vascular endothelial integrity (15).
- Christoffersen, C. and L.B. Nielsen (2013) Curr. Opin. Lipidol. May 6 Epub. PMID 23652568.
- Arkensteijn, B.W. et al. (2013) Int. J. Mol. Sci. 14:4419.
- Xu, N. and B. Dahlback (1999) J. Biol. Chem. 274:31286.
- Zhang X.Y. et al. (2003) Acta Histochem. 105:67.
- Christoffersen, C. et al. (2008) J. Biol. Chem. 283:18765.
- Faber, K. et al. (2006) Mol. Endocrinol. 20:212.
- Christoffersen, C. et al. (2010) Circ. Res. 106:1624.
- Christoffersen, C. et al. (2012) J. Lipid Res. 53:2198.
- Christoffersen, C. et al. (2008) J. Biol. Chem. 283:1839.
- Christoffersen, C. et al. (2006) J. Lipid Res. 47:1833.
- Mulya, A. et al. (2010) J. Lipid Res. 51:514.
- Wolfrum, C. et al. (2005) Nat. Med. 11:418.
- Elsoe, S. et al. (2012) Atherosclerosis 221:91.
- Ahnstrom, J. et al. (2007) J. Lipid Res. 48:1754.
- Christoffersen, C. et al. (2011) Proc. Natl. Acad. Sci. USA 108:9613.
Citation for Recombinant Human Apolipoprotein M Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Apolipoprotein M Inhibits Angiogenic and Inflammatory Response by Sphingosine 1-Phosphate on Retinal Pigment Epithelium Cells
Authors: R Terao, M Honjo, M Aihara
Int J Mol Sci, 2017;19(1):.
Sample Types: Whole Cells
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