Recombinant Human ASIP/Agouti Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived His23-Cys132
  • Accession #
  • N-terminal Sequence
    Analysis
    His23
  • Predicted Molecular Mass
    12 kDa
  • SDS-PAGE
    18-23 kDa, reducing conditions
9094-AG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human ASIP/Agouti (Catalog # 9094-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.
Background: ASIP
Agouti, also known as Agouti Signaling Protein (ASIP), is a 132 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature human Agouti shares 78% and 76% amino acid sequence identity with mouse and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Agouti is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).
  • References:
    1. Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
    2. Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
    3. Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
    4. Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
    5. Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
    6. Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
    7. Lu, D. et al. (1994) Nature 371:799.
    8. Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
    9. Kiefer, L.L. et al. (1997) Biochemistry 36:2084.
  • Long Name:
    Agouti Signaling Protein
  • Entrez Gene IDs:
    434 (Human); 50518 (Mouse); 24152 (Rat)
  • Alternate Names:
    agouti (mouse)-signaling protein; agouti signaling protein; agouti signaling protein, nonagouti homolog (mouse); agouti signaling protein, nonagouti homolog; Agouti switch protein; agouti-signaling protein; AGSW; AGTI; AGTIL; AGTILMGC126092; ASIP; ASPAGSW; MGC126093; nonagouti homolog; SHEP9
Related Research Areas

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