Recombinant Human B7-2/CD86 His Tag Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human B7-2/CD86 is coated at 0.5 µg/mL, Recombinant Human CTLA-4 Fc Chimera  (Catalog # 7268-CT) binds with a typical ED50 of 7-42 ng/mL.
  • Source
    Human embryonic kidney cell, HEK293-derived Leu26-Pro247, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu26
  • Predicted Molecular Mass
    26 kDa
  • SDS-PAGE
    57-66 kDa, reducing conditions
9090-B2
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Human B7-2/CD86 (9090-B2) is coated at 0.5 µg/mL, Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) binds with a typical ED50 of
7-42 ng/mL.
Background: B7-2/CD86
B7-2, also known as CD86, B70, and ETC-1, is a 60-100 kDa variably glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance (1, 2). Mature human B7-2 consists of a 224 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 61 aa cytoplasmic tail (3, 4). Within the ECD, human B7-2 shares 59% aa sequence identity with mouse and rat B7-2. Alternative splicing of human B7-2 generates additional isoforms that lack both Ig-like domains or a region that includes the transmembrane segment. B7-2 is highly expressed on activated antigen presenting cells (APC), e.g. B cells, dendritic cells, and monocytes (4-7), as well as on vascular endothelial cells (8). B7-2 and the closely related B7-1/CD80 exhibit overlapping but distinct functional properties. Their binding to CD28, which is constitutively expressed on T cells, enhances T cell receptor signaling and also provides TCR-independent co-stimulation (3-5, 7, 9-11). B7-1 and B7-2 additionally bind the CD28-related protein, CTLA-4, which is up-regulated and recruited to the immunological synapse (IS) at the onset of T cell activation (3-5, 7, 9, 10). CTLA-4 ligation inhibits the T cell response and supports regulatory T cell function (12). B7-2 is expressed earlier than B7-1 following APC activation (6), and both proteins bind with higher affinity to CTLA-4 than to CD28 (10). B7-2 promotes the stabilization of CD28 in the IS, while B7-1 is primarily responsible for promoting CTLA-4 recruitment and accumulation in the IS (13). The relative participation of B7-1 and B7-2 in T cell co-stimulation can also alter the Th1/Th2 bias of the immune response (14). Both B7-1 and B7-2 serve as cellular receptors for B species adenoviruses (15).
  • References:
    1. Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515.
    2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
    3. Freeman, G.J. et al. (1993) Science 262:909.
    4. Azuma, M. et al. (1993) Nature 366:76.
    5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
    6. Lenschow, D.J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11054.
    7. Hathcock, K.S. et al. (1993) Science 262:905.
    8. Seino, K. et al. (1995) Int. Immunol. 7:1331.
    9. Chen, C. et al. (1994) J. Immunol. 152:4929.
    10. Lanier, L.L. et al. (1995) J. Immunol. 154:97.
    11. Rudd, C.E. et al. (2009) Immunol. Rev. 229:12.
    12. Wing, K. et al. (2011) Trends Immunol. 32:428.
    13. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
    14. Kuchroo, V.K. et al. (1995) Cell 80:707.
    15. Short, J.J. et al. (2006) Virus Res. 122:144.
  • Entrez Gene IDs:
    942 (Human); 12524 (Mouse); 56822 (Rat); 102147235 (Cynomolgus Monkey)
  • Alternate Names:
    Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86
Related Research Areas
Primary Antibodies
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

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His Tag APC-conjugated Antibody

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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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