Recombinant Human Calcium-sensing R/CaSR Protein, CF

Catalog # Availability Size / Price Qty
9608-CS-050
The Innovator Series.
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Recombinant Human Calcium-sensing R/CaSR Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Human embryonic kidney cell, HEK293-derived human Calcium-sensing R/CaSR protein
Tyr20-Lys601, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Tyr20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66 kDa
SDS-PAGE
90-110 kDa, reducing conditions

Product Datasheets

9608-CS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

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Background: Calcium-sensing R/CaSR

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

References
  1. Geng, Y. et al. (2016) Elife 5:e13662.
  2. Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
  3. Brown, E. M. et al. (1993) Nature 366:575.
  4. Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.
Long Name
Calcium-sensing Receptor
Entrez Gene IDs
846 (Human); 12374 (Mouse); 24247 (Rat)
Alternate Names
calcium-sensing receptor; CAR; CaSR; EIG8; extracellular calcium-sensing receptor; FHH; FIH; GPRC2A; GPRC2AHHC; HHC1; hypocalciuric hypercalcemia 1; NSHPT; parathyroid Ca(2+)-sensing receptor 1; Parathyroid cell calcium-sensing receptor; PCaR1; PCAR1MGC138441

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