Recombinant Human Calcium-sensing R/CaSR Protein, CF

R&D Systems | Catalog # 9608-CS

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human Calcium-sensing R/CaSR Protein (9608-CS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P41180-1

Structure / Form

Disulfide-linked homodimer
View all Calcium-sensing R/CaSR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Calcium-sensing R/CaSR protein
Tyr20-Lys601, with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr20

Predicted Molecular Mass

66 kDa

SDS-PAGE

90-110 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9608-CS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Calcium-sensing R/CaSR

Calcium-sensing receptor (CasR) is a plasma membrane G-protein-coupled receptor that senses changes in the extracellular concentration of calcium ions and plays a key role in maintaining calcium homeostasis (1). CasR is expressed in the parathyroid hormone-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule (2). Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism (3). The full length of CasR has a large extracellular N-terminal domain, a central region of 7 transmembrane domains, and a long intracellular C‑terminal domain. The amino acid 20-601 contains the extracellular domain with multiple glycosylation sites (4). Human CasR shares 96.1% and 96.5% aa sequence identity with mouse and rat CasR, respectively.

References

  1. Geng, Y. et al. (2016) Elife 5:e13662.
  2. Hendy, G. N. et al. (2000) Hum. Mutat. 16:281.
  3. Brown, E. M. et al. (1993) Nature 366:575.
  4. Garrett, J. E.et al. (1995) J. Biol. Chem. 270:12919.

Long Name

Calcium-sensing Receptor

Alternate Names

CAR, CaSR, EIG8, FHH, FIH, GPRC2A, HHC1, NSHPT, PCaR1

Entrez Gene IDs

846 (Human); 12374 (Mouse); 24247 (Rat)

Gene Symbol

CASR

UniProt

P41180-1

Additional Calcium-sensing R/CaSR Products

Product Documents for Recombinant Human Calcium-sensing R/CaSR Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Calcium-sensing R/CaSR Protein, CF

For research use only

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