Recombinant Human Caspr2 His-tag Protein, CF
Recombinant Human Caspr2 His-tag Protein, CF Summary
- R&D Systems NS0-derived Recombinant Human Caspr2 His-tag Protein (8207-CRB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Ala28-Ala1262, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8207-CRB
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human Caspr2 His-tag (Catalog # 8207-CRB ) has a molecular weight (MW) of 170.2 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).
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Measured by its binding ability in a functional ELISA. Recombinant Human Caspr2 His-tag Protein (Catalog # 8207-CRB) binds to Recombinant Human Contactin-2/TAG1 Protein ( 1714-CN) with an ED50 of 0.400-4.00 µg/mL.
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2 μg/lane of Recombinant Human Caspr2 His-tag Protein (Catalog # 8207-CRB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa, under reducing conditions.
Reconstitution Calculator
Background: Caspr2
Contact-associated Protein-like 2 (Caspr2) is a type I transmembrane member of the neurexin family of adhesion molecules (1). Human Caspr2 is the vertebrate homolog to Drosophila Neurexin IV, and the human and mouse Caspr2 orthologs share 94% amino acid sequence identity. Human Caspr2 contains two EGF-like domains, one F5/8 type C domain, one fibrinogen C-terminal domain, and four laminin G-like domains. It is highly expressed in neuronal tissue where it is primarily localized to the juxtaparanodal region of the axonal membrane (1, 2). Caspr2 acts in conjunction with 4.1B and Tag-1 as a scaffold that clusters Kv1 potassium channels at the juxtaparanodal region and is critical for axo-glial contacts (1-6). Caspr2 interacts with carboxypeptidase E in Golgi bodies during intracellular trafficking to the cell membrane (7). Caspr2 is also required for dendrite arborization and the normal development of neural networks (8). Mutations in Caspr2 are associated with predisposition to autism spectrum disorders, epilepsy, attention-deficit hyperactive disorder, and schizophrenia (8-11). The presence of autoantibodies against Caspr2 is associated with encephalitis, epilepsy, dysarthria, and paroxysmal kinesigenic dystonia (12-14). R&D Systems in-house testing indicates that Caspr2 can enhance bovine corneal endothelial cell adhesion.
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- Anderson, G.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:18120.
- Penagarikano, O. and D.H. Geschwind (2012) Trends Mol. Med. 18:156.
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- Lancaster, E. et al. (2011) Ann. Neurol. 69:303.
- Balint, B. et al. (2013) J. Neurol. Sci. 327:73.
- Krogias, C. et al. (2013) JAMA Neurol. 70:1056.
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