Recombinant Human Caspr2 His-tag Protein, CF

R&D Systems | Catalog # 8207-CRB

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human Caspr2 His-tag Protein (8207-CRB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9UHC6.1

Applications

Bioactivity
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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Caspr2 protein
Ala28-Ala1262, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala 28

Predicted Molecular Mass

138 kDa

SDS-PAGE

130-150 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Caspr2 His-tag binds to Recombinant Human Contactin-2/TAG1 Protein (Catalog # 1714-CN) with an ED50 of 0.400-4.00 μg/mL.

Scientific Data Images for Recombinant Human Caspr2 His-tag Protein, CF

Recombinant Human Caspr2 His-tag Protein SEC-MALS.

Recombinant Human Caspr2 His-tag (Catalog # 8207-CRB ) has a molecular weight (MW) of 170.2 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (e.g. Glycosylation).

Recombinant Human Caspr2 His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Recombinant Human Caspr2 His-tag Protein (Catalog # 8207-CRB) binds to Recombinant Human Contactin-2/TAG1 Protein ( 1714-CN) with an ED50 of 0.400-4.00 µg/mL.

Recombinant Human Caspr2 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Caspr2 His-tag Protein (Catalog # 8207-CRB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 130-150 kDa, under reducing conditions.

Formulation, Preparation, and Storage

8207-CRB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 100 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caspr2

Contact-associated Protein-like 2 (Caspr2) is a type I transmembrane member of the neurexin family of adhesion molecules (1). Human Caspr2 is the vertebrate homolog to Drosophila Neurexin IV, and the human and mouse Caspr2 orthologs share 94% amino acid sequence identity. Human Caspr2 contains two EGF-like domains, one F5/8 type C domain, one fibrinogen C-terminal domain, and four laminin G-like domains. It is highly expressed in neuronal tissue where it is primarily localized to the juxtaparanodal region of the axonal membrane (1, 2). Caspr2 acts in conjunction with 4.1B and Tag-1 as a scaffold that clusters Kv1 potassium channels at the juxtaparanodal region and is critical for axo-glial contacts (1-6). Caspr2 interacts with carboxypeptidase E in Golgi bodies during intracellular trafficking to the cell membrane (7). Caspr2 is also required for dendrite arborization and the normal development of neural networks (8). Mutations in Caspr2 are associated with predisposition to autism spectrum disorders, epilepsy, attention-deficit hyperactive disorder, and schizophrenia (8-11). The presence of autoantibodies against Caspr2 is associated with encephalitis, epilepsy, dysarthria, and paroxysmal kinesigenic dystonia (12-14). R&D Systems in-house testing indicates that Caspr2 can enhance bovine corneal endothelial cell adhesion.

References

  1. Poliak, S. et al. (1999) Neuron 24:1037.
  2. Poliak, S. et al. (2001) J. Neurosci. 21:7568.
  3. Horresh, I. et al. (2010) J. Neurosci. 30:2480.
  4. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
  5. Denisenko-Nehrbass, N. et al. (2003) Eur. J. Neurosci. 17:411.
  6. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.
  7. Oiso, S. et al. (2009) J. Neurochem. 109:158.
  8. Anderson, G.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:18120.
  9. Penagarikano, O. and D.H. Geschwind (2012) Trends Mol. Med. 18:156.
  10. Friedman, J.I. et al. (2008) Mol. Psychiatry 13:261.
  11. Elia, J. et al. (2010) Mol. Psychiatry 15:637.
  12. Lancaster, E. et al. (2011) Ann. Neurol. 69:303.
  13. Balint, B. et al. (2013) J. Neurol. Sci. 327:73.
  14. Krogias, C. et al. (2013) JAMA Neurol. 70:1056.

Long Name

Contactin Associated Protein-like 2

Alternate Names

CDFE, CNTNAP2, NRXN4

Entrez Gene IDs

26047 (Human); 66797 (Mouse)

Gene Symbol

CNTNAP2

UniProt

Q9UHC6.1

Additional Caspr2 Products

Product Documents for Recombinant Human Caspr2 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human Caspr2 His-tag Protein, CF

For research use only

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