Recombinant Human CCL3/MIP-1 alpha Protein

Carrier Free

Catalog # Availability Size / Price Qty
270-LD-010/CF
270-LD-050/CF

With Carrier

Catalog # Availability Size / Price Qty
270-LD-010
270-LD-050
Recombinant Human CCL3/MIP-1 alpha Protein Bioactivity
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Product Details
Citations (37)
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Recombinant Human CCL3/MIP-1 alpha Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to chemoattract 2-day cultured human monocytes. The ED50 for this effect is 2‑10 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 3‑10 ng/mL.
Source
E. coli-derived human CCL3/MIP-1 alpha protein
Ala27-Ala92
Accession #
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
7.5 kDa

Product Datasheets

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270-LD (with carrier)

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270-LD/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

270-LD

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

270-LD/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human CCL3/MIP-1 alpha Protein Bioactivity View Larger

Recombinant Human CCL3/MIP-1 alpha Isoform LD78a (Catalog # 270-LD) chemoattracts the BaF3 mouse pro-B cells transfected with human CCR5. The ED50 for this effect is 3-10 ng/mL.

SDS-PAGE Recombinant Human CCL3/MIP-1 alpha Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human CCL3/MIP-1 alpha Isoform LD78a was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 12 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature human CCL3 shares 70% - 74% amino acid sequence identity with mouse, rat, and cotton rat CCL3 (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8 - 10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11 - 14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).

References
  1. Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
  2. Obaru, K. et al. (1986) J. Biochem. 99:885.
  3. Graham, G.J. et al. (1990) Nature 344:442.
  4. Wagner, L. et al. (1998) Nature 391:908.
  5. Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
  6. Ren, M. et al. (2010) EMBO J. 29:3952.
  7. Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
  8. Taub, D.D. et al. (1993) Science 260:355.
  9. Bernardini, G. et al. (2008) Blood 111:3626.
  10. Lee, S.C. et al. (2000) J. Immunol. 164:3392.
  11. Wu, Y. et al. (2008) J. Immunol. 181:6384.
  12. Cook, D.N. et al. (1995) Science 269:1583.
  13. Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
  14. Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
  15. Cocchi, F. et al. (1995) Science 270:1811.
  16. Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.
Entrez Gene IDs
6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)
Alternate Names
C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

Citations for Recombinant Human CCL3/MIP-1 alpha Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. A Polymorphism in C-C Chemokine Receptor 5 (CCR5) Associates with L�fgren's Syndrome and Alters Receptor Expression as well as Functional Response
    Authors: B Karakaya, CHM van Moorse, M Veltkamp, C Roodenburg, KM Kazemier, AHM van der He, TWJ Huizinga, JC Grutters, GT Rijkers
    Cells, 2021;10(8):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Prevention of CaCl2-induced aortic inflammation and subsequent aneurysm formation by the CCL3-CCR5 axis
    Authors: Y Ishida, Y Kuninaka, M Nosaka, A Kimura, A Taruya, M Furuta, N Mukaida, T Kondo
    Nat Commun, 2020;11(1):5994.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. CCL3-CCR5 axis contributes to progression of esophageal squamous cell carcinoma by promoting cell migration and invasion via Akt and ERK pathways
    Authors: T Kodama, YI Koma, N Arai, A Kido, N Urakawa, M Nishio, M Shigeoka, H Yokozaki
    Lab. Invest., 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. GWAS-identified CCR1 and IL10 loci contribute to M1 macrophage-predominant inflammation in Beh�et's disease
    Authors: H Nakano, Y Kirino, M Takeno, K Higashitan, H Nagai, R Yoshimi, Y Yamaguchi, I Kato, I Aoki, H Nakajima
    Arthritis Res. Ther., 2018;20(1):124.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. BTKCys481Serdrives ibrutinib resistance via ERK1/2, and protects BTKWild-TypeMYD88 mutated cells by a paracrine mechanism
    Authors: JG Chen, X Liu, M Munshi, L Xu, N Tsakmaklis, MG Demos, A Kofides, ML Guerrera, GG Chan, CJ Patterson, KE Meid, J Gustine, T Dubeau, P Severns, JJ Castillo, ZR Hunter, J Wang, SJ Buhrlage, NS Gray, SP Treon, G Yang
    Blood, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Macrophages confer survival signals via CCR1-dependent translational MCL-1 induction in chronic lymphocytic leukemia
    Authors: MH van Atteku, S Terpstra, E Slinger, M von Linder, PD Moerland, A Jongejan, AP Kater, E Eldering
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Molecular Mechanism of Action for Allosteric Modulators and Agonists in CC-chemokine Receptor 5 (CCR5)
    Authors: Stefanie Karlshøj
    J. Biol. Chem, 2016;291(52):26860-26874.
    Applications: Bioassay
  8. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies
    Mucosal Immunol, 2016;9(6):1584-1595.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study
    Authors: Benjamin Malard
    Artif Organs, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: Hemofiltration Procedure
  10. Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.
    Authors: Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J
    Eur J Immunol, 2015;45(1):101-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Increased levels of soluble interleukin-6 receptor and CCL3 in COPD sputum.
    Authors: Ravi A, Khurana S, Lemon J, Plumb J, Booth G, Healy L, Catley M, Vestbo J, Singh D
    Respir Res, 2014;15(0):103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Thymoquinone inhibits the CXCL12-induced chemotaxis of multiple myeloma cells and increases their susceptibility to Fas-mediated apoptosis.
    Authors: Badr G, Lefevre EA, Mohany M
    PLoS ONE, 2011;6(9):e23741.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. CXCR4 and CCR5 ligands cooperate in monocyte and lymphocyte migration and in inhibition of dual-tropic (R5/X4) HIV-1 infection.
    Authors: Gouwy M, Struyf S, Berghmans N, Vanormelingen C, Schols D, Van Damme J
    Eur. J. Immunol., 2011;41(4):963-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. CCL19 is a specific ligand of the constitutively recycling atypical human chemokine receptor CRAM-B.
    Authors: Leick M, Catusse J, Follo M, Nibbs RJ, Hartmann TN, Veelken H, Burger M
    Immunology, 2010;129(4):536-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010;56(0):1320.
  17. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
    Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
    Int. J. Cancer, 2008;122(4):769-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Regulation of D6 chemokine scavenging activity by ligand- and Rab11-dependent surface up-regulation.
    Authors: Bonecchi R, Borroni EM, Anselmo A, Doni A, Savino B, Mirolo M, Fabbri M, Jala VR, Haribabu B, Mantovani A, Locati M
    Blood, 2008;112(3):493-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
    Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
    AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  20. Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes.
    Authors: Nobile C, Lind M, Miro F, Chemin K, Tourret M, Occhipinti G, Dogniaux S, Amigorena S, Hivroz C
    Blood, 2008;111(7):3579-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008;84(4):1109-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Regulation of human LZIP expression by NF-kappaB and its involvement in monocyte cell migration induced by Lkn-1.
    Authors: Jang SW, Kim YS, Kim YR, Sung HJ, Ko J
    J. Biol. Chem., 2007;282(15):11092-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  24. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
    Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
    Blood, 2007;109(10):4272-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  25. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  26. MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma.
    Authors: Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK
    Blood, 2006;108(10):3465-71.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  27. Novel antiviral activity of chemokines.
    Authors: Nakayama T, Shirane J, Hieshima K, Shibano M, Watanabe M, Jin Z, Nagakubo D, Saito T, Shimomura Y, Yoshie O
    Virology, 2006;350(2):484-92.
    Species: Virus
    Sample Types: Virus
    Applications: Binding Assay
  28. Interaction between the CCR5 chemokine receptors and microbial HSP70.
    Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
    Eur. J. Immunol., 2006;36(9):2304-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Changes in soluble factor-mediated CD8+ cell-derived antiviral activity in cynomolgus macaques infected with simian immunodeficiency virus SIVmac251: relationship to biological markers of progression.
    Authors: Dioszeghy V, Benlhassan-Chahour K, Delache B, Dereuddre-Bosquet N, Aubenque C, Gras G, Le Grand R, Vaslin B
    J. Virol., 2006;80(1):236-45.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  30. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004;78(16):8720-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
    Authors: Tay SS, McCormack A, Rose ML
    Transplantation, 2004;78(7):987-94.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  33. Distribution, cellular localization and functional role of CCR2 chemokine receptors in adult rat brain.
    Authors: Banisadr G, Queraud-Lesaux F, Boutterin MC, Pelaprat D, Zalc B, Rostene W, Haour F, Parsadaniantz SM
    J. Neurochem., 2002;81(2):257-69.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Binding Assay
  34. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  35. Induction of inhibitory antibodies to the CCR5 chemokine receptor and their complementary role in preventing SIV infection in macaques.
    Authors: Lehner T, Wang Y, Doyle C, Tao L, Bergmeier LA, Mitchell E, Bogers WM, Heeney J, Kelly CG
    Eur. J. Immunol., 1999;29(8):2427-35.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Neuropeptide Y induces potent migration of human immature dendritic cells and promotes a Th2 polarization.
    Authors: Buttari B, Profumo E, Domenici G, Tagliani A, Ippoliti F, Bonini S, Businaro R, Elenkov I, Rigano R
    FASEB J, 0;28(7):3038-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
    Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
    iScience, 0;24(12):103433.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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