Recombinant Human CD151 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1884-CD-050
Recombinant Human CD151 Fc Chimera Protein, CF Binding Activity
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Recombinant Human CD151 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Integrin  alpha V beta 3 (Catalog # 3050-AV) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD151 Fc Chimera binds with an ED50 of 0.3-1.8 μg/mL.  
Source
Chinese Hamster Ovary cell line, CHO-derived human CD151 protein
MDHuman IgG1
(Pro100-Lys330)
IEGR Human CD151-LEL
(Ala113-Arg221)
Accession # P48509
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
39 kDa
SDS-PAGE
41-55, and 90-102 kDa (non-reducible dimer), reducing conditions

Product Datasheets

1884-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Human CD151 Fc Chimera Protein, CF Binding Activity View Larger

When Recombinant Human Integrin  alpha V beta 3 (Catalog # 3050-AV) isimmobilized at 1 μg/mL, 100 μL/well, Recombinant Human CD151 Fc Chimera bindswith an ED50 of 0.3‑1.8 μg/mL.

SDS-PAGE Recombinant Human CD151 Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human CD11b/Integrin alpha M was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 41-55 kDa and 80 - 110 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: CD151

Human CD151, also known as SFA-1, Tetraspanin-24, and GP27, is a palmitoylated glycoprotein in the tetraspanin superfamily. It is the first tetraspanin member to be identified as a promoter of cancer metastasis (1, 2), and it is found to participate in nearly all stages of cancer progression (3). CD-151 is normally expressed in endothelial cells, platelets, and frequently over-expressed in cancer cells (4). Mature CD-151 is a multi-pass membrane protein that contains four transmembrane domains, three cytoplasmic domains, and two extracellular loops. The region of amino acids (aa) 113-221 contains the largest extracellular loop (LEL) that involves in interacting with integrins and regulating integrin functions (5). Human CD151 LEL shares 88.9% and 87.0% aa identity with that of mouse and rat respectively. CD151 interacts with integrins such as alpha v beta 3, alpha 3 beta 1, alpha 6 beta 1, alpha 7 beta 1, and alpha 6 beta 4 to regulate their activities and thus resulting in modulation of adhesion, spreading, migration, angiogenesis, invasion and metastasis (1, 3-5). CD151 can also complex with immunoglobulin super family proteins and other tetraspanins such as CD9, CD81, and CD63 (3). Clinically, high levels of CD151 are correlated with poor prognosis in a variety of tumors (3, 4). CD151 has been implicated as a potential diagnostic marker in osteosarcoma and prostate cancer and a putative target for antibody-based immunotherapy (3). CD-151 is a key player in the formation of basement membranes in kidney and skin tissues; it is also associated with human papillomavirus (HPV) infection (6, 7).

References
  1. Detchokul, S. et al. (2014) Br. J. Pharmacol. 171(24):5462.
  2. Hasegawa, H. et al. (1996). Journal of Virology 70: 3258.
  3. Sadej, R. et al. (2014) Laboratory Investigation 94:41.
  4. Kumari, S. et al. (2015) Biomark Cancer 7:7.
  5. Yu J. et al. (2017) Biochem. J. 474(4):589.
  6. Scheffer, K.D. et al. (2014) Viruses 6:893.
  7. Karamatic Crew, V. et al. (2004) Blood 104:2217.
Entrez Gene IDs
977 (Human); 12476 (Mouse); 64315 (Rat)
Alternate Names
CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151

FAQs

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Primary Antibodies

Human IgG1 Antibody

Human IgG Fc Antibody

WB,Flow,CyTOF-ready

Recombinant Proteins

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