Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI9174-050

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Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein Binding Activity
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Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein, CF Summary

Why choose R&D Systems Avi-tag CD155 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) binds with an ED50 of 10-60 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human CD155/PVR protein
Human CD155/PVR
(Asp28-Asn343)
Accession # NP_006496.4
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp28
Structure / Form
Disulfide-linked homodimer, Biotinylated via Avi-tag
Predicted Molecular Mass
63 kDa
SDS-PAGE
86-98 kDa, reducing conditions

Product Datasheets

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AVI9174

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9174

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein Binding Activity View Larger

When Recombinant Human CD96v2 Fc Chimera (Catalog # 9556-CD) is immobilized at 1 µg/mL, Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) binds with an ED50 of 10-60 ng/mL.

SDS-PAGE Recombinant Human CD155/PVR Fc Chimera Avi-tag Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (Catalog # AVI9174) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 86-98 kDa and 172-200 kDa, respectively.

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Background: CD155/PVR

CD155, also known as PVR (poliovirus receptor), Necl-5 (nectin-like molecule-5) and, in rodents, TAGE4 (tumor-associated glycoprotein E4), is a 70-kDa type I transmembrane glycoprotein in the nectin-related family of adhesion proteins within the immunoglobulin superfamily (1, 2). CD155 binds other molecules including Vitronectin, Nectin-3, DNAM-1/CD226, CD96, and TIGIT but does not bind homotypically (3). Mature human CD155 consists of a 323 amino acid (aa) extracellular domain (ECD) with one N-terminal V-type and two C2-type Ig-like domains, a 24 aa transmembrane segment, and a 50 aa cytoplasmic tail. Within the ECD, human CD155 shares 45% aa sequence identity with mouse and rat CD155, and 52% with human Nectin-2. The V-type domain of CD155 mediates all binding, including to polio virus (1), and alternative splicing within this domain in humans can modulate ligand binding (4). Human CD155 can also be spliced to generate secreted isoforms (5). CD155 is up-regulated on endothelial cells by IFN-gamma and is highly expressed on immature thymocytes, lymph node dendritic cells, and tumor cells of epithelial and neuronal origin (1, 2, 6-9). It is preferentially expressed on Th17 cells compared to Th2 cells (10), and its activation promotes the development of Th1 responses (11). On migrating cells, CD155 is concentrated at the leading edge, where it binds basement membrane Vitronectin, recruits Nectin-3-expressing cells, and cooperates with PDGF and Integrin alpha v beta 3 to promote cell migration (1, 3, 12). Enhanced CD155 expression in tumor cells contributes to loss of contact inhibition and increased migration but also allows tumor cell recognition and killing by DNAM-1 or CD96 expressing NK cells (1, 7, 13). Binding of monocyte DNAM-1 to endothelial cell CD155 promotes transendothelial migration (8). The expression of CD155 on mouse CD8+ thymocytes prevents their premature exit from the thymus (14). Within intestinal Peyer's patches, follicular dendritic cell CD155 activates follicular helper T cells via DNAM-1 or CD96 binding (7-9, 15). CD155 also binds the inhibitory ligand TIGIT on NK and some mature T cells, antagonizing DNAM-1 effects (7, 15, 16).

References
  1. Mandai, K. et al. (2015) Curr. Top. Dev. Biol. 112:197.
  2. Mendelsohn, C.L. et al. (1989) Cell 56:855.
  3. Sato, T. et al. (2004) Genes to Cells 9:791.
  4. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  5. Koike, S. et al. (1990) EMBO J. 9:3217.
  6. Escalante, N.K. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:1177.
  7. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
  8. Reymond, N. et al. (2004) J. Exp. Med. 199:1331.
  9. Maier, M.K. et al. (2007) Eur. J. Immunol. 37 :2214.
  10. Lozano, E. et al. (2013) J. Immunol. 191:3673.
  11. Yamashita-Kanemaru, Y. et al. (2015) J. Immunol. 194:5644.
  12. Mueller, S. and E. Wimmer (2003) J. Biol. Chem. 278:31251.
  13. Chan, C.J. et al. (2010) J. Immunol. 184:902.
  14. Qui, Q. et al. (2010) J. Immunol. 184:1681.
  15. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  16. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
Long Name
Poliovirus Receptor
Entrez Gene IDs
5817 (Human); 52118 (Mouse); 25066 (Rat); 102136444 (Cynomolgus Monkey); 712851 (Rhesus Macaque)
Alternate Names
CD155 antigen; CD155; HVED; NECL5; Necl-5; nectin-like 5; Nectin-like protein 5; poliovirus receptor; PVR; PVS; PVSFLJ25946; Tage4

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