Recombinant Human CD19 Fc Chimera Protein, CF

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ELISA chart showing Recombinant Human CD19 binding to human CD81
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Product Details
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Recombinant Human CD19 Fc Chimera Protein, CF Summary

Why choose R&D Systems CD19 Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

NEW! Fluorescent labeled CD19 designed for validating expression of a CD19 chimeric antigen receptor (CAR) Fluorescent CD19 Proteins

Product Specifications

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human CD19 Fc Chimera is immobilized at 2 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 4-20 μg/mL.
Chinese Hamster Ovary cell line, CHO-derived human CD19 protein
Human CD19
Accession # P15391
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
57 kDa
77-92 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity ELISA chart showing Recombinant Human CD19 binding to human CD81 View Larger

When Recombinant Human CD19 Fc Chimera 9269-CD is immobilized at 2 µg/mL to 100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 4-20 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: CD19

CD19 protein is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 is expressed throughout B cell development from pre-B cells through mature B cells, and it is commonly used as a B cell lineage marker (1). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (4, 5). The CD19 protein associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu13, (6-9). These associations enable CD19 to amplify B cell signaling (7, 9-12) and reduce the threshold for antigen stimulation through the BCR (13). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19 has emerged as promising therapeutic target for hematologic cancers and solid tumors. Immunotherapy using a chimeric antigen receptor (CAR) targeting CD19 has proven effective in many different cancers. The first CD19 CAR T cell therapies have been granted FDA approval for the treatment of B cell malignancies with several more in clinical trials.

Mature human CD19 protein consists of a 272 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 22 aa transmembrane segment, and a 243 aa cytoplasmic domain (3). Within the ECD, human CD19 shares 57% amino acid sequence identity with mouse and rat CD19.

  1. Wang, K. et al. (2012) Exp. Hematol. Oncol. 1:36.
  2. Tedder, T.F. et al. (1997) Immunity 6:107.
  3. Tedder, T.F. and C.M. Isaacs (1989) J. Immunol. 143:712.
  4. Rickert, R.C. et al. (1995) Nature 376:352.
  5. Engel, P. et al. (1995) Immunity 3:39.
  6. Vences-Catalan, F. et al. (2012) J. Immunol. 137:48.
  7. Deaglio, S. et al. (2007) Blood 109:5390.
  8. Bradbury, L. et al. (1992) J. Immunol. 149:2841.
  9. Krop, I. et al. (1996) J. Immunol. 157:48.
  10. O'Rourke, L.M. et al. (1998) Immunity 8:635.
  11. Buhl, A.M. et al. (1997) J. Exp. Med. 186:1897.
  12. Sato, S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11558.
  13. Carter, R.H. and D.T. Fearon (1992) Science 256:105.
Entrez Gene IDs
930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)
Alternate Names
B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Citations for Recombinant Human CD19 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Murine CAR19 Tregs suppress acute graft-versus-host disease and maintain graft-versus-tumor responses
    Authors: S Bolivar-Wa, ML Loschi, S Jin, G Thangavelu, JH Larson, CS McDonald-H, EA Aguilar, A Saha, BH Koehn, M Hefazi, MJ Osborn, MC Jensen, JE Wagner, CA Pennell, BR Blazar
    JCI Insight, 2022-09-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A rational approach to assess off-target reactivity of a dual-signal integrator for T cell therapy
    Authors: X Wang, LM Wong, ME McElvain, S Martire, WH Lee, CZ Li, FA Fisher, RL Maheshwari, ML Wu, MC Imun, R Murad, DT Warshaviak, J Yin, A Kamb, H Xu
    Toxicology and Applied Pharmacology, 2022-01-25;0(0):115894.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. HLA-independent T cell receptors for targeting tumors with low antigen density
    Authors: J Mansilla-S, J Eyquem, S Haubner, M Hamieh, J Feucht, N Paillon, AE Zucchetti, Z Li, M Sjöstrand, PL Lindenberg, M Saetersmoe, A Dobrin, M Maurin, A Iyer, A Garcia Ang, MM Miele, Z Zhao, T Giavridis, SJC van der St, F Tamzalit, I Rivière, M Huse, RC Hendrickso, C Hivroz, M Sadelain
    Nature Medicine, 2022-01-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Liposome-based nanocarriers loaded with anthrax lethal factor and armed with anti-CD19 VHH for effectively inhibiting MAPK pathway in B cells
    Authors: SR Banihashem, F Rahbarizad, A Zavaran Ho, D Ahmadvand, S Khoshtinat
    International immunopharmacology, 2021-09-06;100(0):107927.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay


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Recombinant Human CD19 Fc Chimera Protein, CF
By YANYING FAN on 10/07/2020
Application: Binding assay/Protein-protein interaction

Recombinant Human CD19 Fc Chimera Protein, CF
By Jenna Nguyen on 05/07/2019
Application: Media additive for protein or antibody production

Recombinant Human CD19 Fc Chimera Protein, CF
By Jenna Nguyen on 07/27/2018
Application: Apoptosis assay

Recombinant Human CD19 Fc Chimera Protein, CF
By Anonymous on 10/31/2017
Application: Immunoassay Standard