Recombinant Human CD24 Fc Chimera Protein, CF

R&D Systems | Catalog # 5247-CD

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CD24 Fc Chimera Protein (5247-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

NP_037362

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD24 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD24 protein
Human CD24
(Met1 - Gly59)
Accession # NP_037362		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser27

Predicted Molecular Mass

29.7 kDa (monomer)

SDS-PAGE

45-55 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human CD24 Fc Chimera is coated at 5 μg/mL, the concentration of Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) that produces 50% optimal binding response is found to be approximately 0.3-3 μg/mL.

Reviewed Applications

Read 2 reviews rated 5 using 5247-CD in the following applications:

Formulation, Preparation, and Storage

5247-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD24

CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa ‑ 60 kDa GPI-linked sialoprotein (1 - 4). Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells (4 - 6). In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells (2). CD24 expression is regulated during lineage development and with the activation of various cell types (4). Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance (7 - 11). CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells (12 - 14). CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells (15). Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs (15, 16). Mature human CD24 shares 30% and 42% amino acid sequence identity with mouse and rat CD24, respectively.

References

  1. Jackson, D. et al. (1992) Cancer Res. 52:5264.
  2. Kay, R. et al. (1991) J. Immunol. 147:1412.
  3. Kadmon, G. et al. (1992) J. Cell Biol. 118:1245.
  4. Fang, X. et al. (2010) Cell. Mol. Immunol. 7:100.
  5. Fischer, G. F. et al. (1990) J. Immunol. 144:638.
  6. Allman, D.M. et al. (1993) J. Immunol. 151:4431.
  7. Suzuki, T. et al. (2001) J. Immunol. 166:5567.
  8. Taguchi, T. et al. (2003) J. Immunol. 170:252.
  9. Jung, K.C. et al. (2004) J. Immunol. 172:795.
  10. Kishimoto, H. and J. Sprent (1997) J. Exp. Med. 185:263.
  11. Hitsumoto, Y. et al. (1996) Immunology 89:200.
  12. Liu, Y. et al. (1992) Eur. J. Immunol. 22:2855.
  13. Enk, A.H. and S.I. Katz (1994) J. Immunol. 152:3264.
  14. Askew, D. and C.V. Harding (2008) Immunology 123:447.
  15. Chen G.Y. et al. (2009) Science 323:1722.
  16. Liu, Y. et al. (2011) Curr. Opin. Immunol. 23:41.

Alternate Names

CD24, CD24A

Entrez Gene IDs

100133941 (Human); 12484 (Mouse)

Gene Symbol

CD24

UniProt

NP_037362

Additional CD24 Products

Product Documents for Recombinant Human CD24 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CD24 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human CD24 Fc Chimera Protein, CF

Customer Reviews for Recombinant Human CD24 Fc Chimera Protein, CF (2)

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  • Recombinant Human CD24 Fc Chimera Protein, CF
    Name: William Jackson
    Application: Immunoassay Standard
    Verified Customer | Posted 03/14/2024
    Recombinant Human CD24 Fc Chimera Protein, CF 5247-CD
  • Recombinant Human CD24 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Adhesion
    Verified Customer | Posted 05/18/2020

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