Recombinant Human CD24 Fc Chimera Protein, CF

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Recombinant Human CD24 Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA.

When Recombinant Human CD24 Fc Chimera is coated at 5 μg/mL, the concentration of Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) that produces 50% optimal binding response is found to be approximately 0.3-3 μg/mL.

Mouse myeloma cell line, NS0-derived human CD24 protein
Human CD24
(Met1 - Gly59)
Accession # NP_037362
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
29.7 kDa (monomer)
45-55 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: CD24

CD24, also known as Heat-Stable Antigen and Nectadrin, is a heavily and variably glycosylated 30 kDa ‑ 60 kDa GPI-linked sialoprotein (1 - 4). Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells (4 - 6). In mouse, CD24 is even more widely expressed, particularly on T cells, monocytes, and dendritic cells (2). CD24 expression is regulated during lineage development and with the activation of various cell types (4). Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance (7 - 11). CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells (12 - 14). CD24 associates in cis with Siglec-10 (or Siglec-G in mouse) and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells (15). Formation of these ternary complexes fills a protective role: the resulting Siglec-10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs (15, 16). Mature human CD24 shares 30% and 42% amino acid sequence identity with mouse and rat CD24, respectively.

  1. Jackson, D. et al. (1992) Cancer Res. 52:5264.
  2. Kay, R. et al. (1991) J. Immunol. 147:1412.
  3. Kadmon, G. et al. (1992) J. Cell Biol. 118:1245.
  4. Fang, X. et al. (2010) Cell. Mol. Immunol. 7:100.
  5. Fischer, G. F. et al. (1990) J. Immunol. 144:638.
  6. Allman, D.M. et al. (1993) J. Immunol. 151:4431.
  7. Suzuki, T. et al. (2001) J. Immunol. 166:5567.
  8. Taguchi, T. et al. (2003) J. Immunol. 170:252.
  9. Jung, K.C. et al. (2004) J. Immunol. 172:795.
  10. Kishimoto, H. and J. Sprent (1997) J. Exp. Med. 185:263.
  11. Hitsumoto, Y. et al. (1996) Immunology 89:200.
  12. Liu, Y. et al. (1992) Eur. J. Immunol. 22:2855.
  13. Enk, A.H. and S.I. Katz (1994) J. Immunol. 152:3264.
  14. Askew, D. and C.V. Harding (2008) Immunology 123:447.
  15. Chen G.Y. et al. (2009) Science 323:1722.
  16. Liu, Y. et al. (2011) Curr. Opin. Immunol. 23:41.
Entrez Gene IDs
100133941 (Human); 12484 (Mouse)
Alternate Names
CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24; CD24A; CD24Asignal transducer CD24; FLJ22950; FLJ43543; MGC75043; Small cell lung carcinoma cluster 4 antigen


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Recombinant Human CD24 Fc Chimera Protein, CF
By Jenna Nguyen on 05/18/2020
Application: Cell Adhesion