Recombinant Human CD27 Ligand/TNFSF7 Protein, CF

Analyzed by SEC-MALS
Catalog # Availability Size / Price Qty
Recombinant Human CD27 Ligand/TNFSF7 Protein SEC-MALS.
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Recombinant Human CD27 Ligand/TNFSF7 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060).
Human embryonic kidney cell, HEK293-derived human CD27 Ligand/TNFSF7 protein
Accession # P32970.2
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Noncovalently-linked homotrimer
Predicted Molecular Mass
23 kDa
24-32 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human CD27 Ligand/TNFSF7 (Catalog # 9328-CL) has a molecular weight (MW) of 72.4 kDa as analyzed by SEC-MALS, suggesting that this protein is a homotrimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Bioactivity Recombinant Human CD27 Ligand/TNFSF7 Protein Bioactivity View Larger

Recombinant Human CD27 Ligand/TNFSF7 (Catalog # 9328-CL) induces IL-8 secretion in HT1080 human fibrosarcoma cells transfected with Human CD27. The ED50 for this effect is 5-25 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: CD27 Ligand/TNFSF7

CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Human CD27L cDNA encodes a 193 amino acid (aa) residue protein with a 17 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 155 aa C-terminal extracellular domain. Human and mouse CD27L share approximated 56% aa sequence identity. CD27L is expected to exist as non-covalent homotrimers. CD70 is constitutively expressed on medullary thymic epithelial cells (mTECs) in human and mouse and on certain unconventional APCs in the mouse intestine (3). CD70 is also exclusively expressed on DCs (5), B cells (5), conventional- and regulatory T cells (6) and NK cells (7) but only after their activation. CD70 expression is highly regulated by antigen through Pattern Recognition Receptors (PRRs), T cell and B cell antigen receptors (8), and further tuned by cytokines such as IL-1 alpha,
IL-12, TNF alpha, prostaglandin E2 and by CD28 and CD40 co-stimulation (9).  Ligation of CD27 on T cells provides co-stimulatory signals and promotes T cell proliferation, antigen-specific CD4+ and CD8+ T cells clonal expansion, effector & memory T-cell differentiation, and effector T-cell survival & T-cell memory formation (10-12). Ligation of CD27 on mouse B cell and NK cells promotes the B- and NK-cell response by supporting B-cell expansion in the germinal center and increasing IFN-gamma levels in NK cells, in turn enhancing NK cytotoxic capability, which can contribute to tumor control (13,14). Overall, CD27/CD70 co-stimulatory system promote T, B and NK cell responses and particularly the CTL response, and is a target in cancer immunotherapy (15).

  1. Bowman MR. et al. J Immunol. (1994) 152:1756.
  2. Croft, M. (2003) Nature Reviews Immunol. 3:609.
  3. Tesselaar K. et al. (2003) J. Immunol. 170:33.
  4. Sanchez PJ. et al. (2007) J. Immunol. 178:1564.
  5. Lens SM. et al. (1998) Semin. Immunol. 10:491.
  6. Koenen HJ. et al. (2005) J. Immunol. 174:7573.
  7. Hintzen RQ. et al. (1994) Int. Immunol. 6:477.
  8. Borst J. et al. (2005) Curr. Opin. Immunol. 17:275.
  9. Krause P. et al. (2009) Blood 113:2451.
  10. Van Gisbergen K. et al. (2011) Immunity 35:97.
  11. Peperzak V. et al. (2010) J. Immunol. 185:6670.
  12. Pen JJ. et al. (2013) J. Immunol. 191:1976.
  13. Shin CA, et al. (2016) Oncotarget 7:46173.
  14. Takeda K. et al. (2000) J. Immunol. 164:1741.
  15. Wajant H. (2016) Expert Opin Ther Targets 20:959.
Entrez Gene IDs
970 (Human); 21948 (Mouse); 301132 (Rat)
Alternate Names
CD27 Ligand; CD27-L; CD27LCD27 ligand; CD27LG; CD70 molecule; CD70; Ki-24 antigen; surface antigen CD70; TNFSF7; TNFSF7CD70 antigen; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7


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Recombinant Human CD27 Ligand/TNFSF7 Protein, CF
By Anonymous on 11/30/2018
Application: Immunoassay Standard