Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

Name: Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI10380
Price: 592 USD
Availability: InStock

R&D Systems | Catalog # AVI10380

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein (AVI10380)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P25942.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD40/TNFRSF5 Proteins and Enzymes »

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD40/TNFRSF5 protein

Human CD40/TNFRSF5
(Glu21-Arg193)
Accession # P25942.1

IEGRMD

Human IgG₁
(Pro100-Lys330)

Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

48 kDa

SDS-PAGE

61-66 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD40 Ligand/TNFSF5 Fc (HEK293-expressed, (Catalog # 6420-CL) mobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL.

Scientific Data Images for Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein, CF

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein Binding Activity

When Recombinant Human CD40 Ligand/TNFSF5 (HEK293-expressed) Protein (Catalog # 6420-CL) is immobilized at 2 µg/mL, 100 µL/well, the concentration of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) that produces 50% optimal binding is 0.0400-0.320 μg/mL.

Recombinant Human CD40/TNFRSF5 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human CD40/TNFRSF5 Fc Chimera Avi-tag (Catalog # AVI10380) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-66 kDa and 120-135 kDa, respectively.

Formulation, Preparation, and Storage

AVI10380

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).