Recombinant Human CD74 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human CD74 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells.

When 5 x 104 cells per well are added to Recombinant Human CD74 coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.6-3 μg/mL.

Optimal dilutions should be determined by each laboratory for each application.
Chinese Hamster Ovary cell line, CHO-derived human CD74 protein
Gln73-Met232, with an N-terminal HA (YPYDVPDYA) tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
19.3 kDa
30-35 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD74

CD74, also known as Invariant chain (Ii) and p33, is a type 2 transmembrane glycoprotein that plays an important role in adaptive immunity, inflammation, and cancer (1). Mature human CD74 consists of a 46 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 224 aa extracellular domain (ECD) that contains one thyroglobulin type 1 domain (2, 3). Alternate splicing and the use of a second initiation start site result in the synthesis of additional isoforms (ranging from 31 kDa ‑ 41 kDa) that differ by the presence or absence of a 16 aa N‑terminal extension and/or a 64 aa internal section of the ECD (4). This recombinant protein does not contain the 64 aa insertion. Within comparable regions of the ECD, human CD74 shares 70% and 73% aa sequence identity with mouse and rat CD74, respectively. CD74 functions as a chaperone for MHC class II molecules on antigen presenting cells and undergoes progressive proteolysis during class II trafficking and antigenic peptide loading (5). Full length CD74 assembles into trimers which then associate with class II molecules in nonameric complexes on the cell surface (6, 7). CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up‑regulation of angiogenic factors in endometrial stromal cells (8 ‑ 13). MIF binding notably induces the proteolytic cleavage of the CD74 intracellular domain which then promotes B cell differentiation (12). CD74 is up‑regulated on non‑immune cells at sites of inflammation including amyloid beta plaques and atherosclerotic plaques (14, 15). It is also up‑regulated in a variety of cancers and enhances tumorigenicity, tumor angiogenesis, and metastasis (1, 16).

  1. Beswick, E.J. and V.E. Reyes (2009) World J. Gastroenterol. 15:2855.
  2. Claesson, L. et al. (1983) Proc. Natl. Acad. Sci. 80:7395.
  3. Strubin, M. et al. (1984) EMBO J. 3:869.
  4. Strubin, M. et al. (1986) EMBO J. 5:3483.
  5. Riberdy, J.M. et al. (1992) Nature 360:474.
  6. Koch, N. et al. (1991) J. Immunol. 147:2643.
  7. Roche, P.A. et al. (1991) Nature 354:392.
  8. Leng, L. et al. (2003) J. Exp. Med. 197:1467.
  9. Takahashi, K. et al. (2009) Respir. Res. 10:33.
  10. Heinrichs, D. et al. (2011) Proc. Natl. Acad. Sci. 104:17444.
  11. Shi, X. et al. (2006) Immunity 25:595.
  12. Gore, Y. et al. (2008) J. Biol. Chem. 283:2784.
  13. Veillat, V. et al. (2010) J. Clin. Endocrinol. Metab. 95:E403.
  14. Bryan, K.J. et al. (2008) Mol. Neurodegen. 3:13.
  15. Martin-Ventura, J.L. et al. (2009) Cardiovasc. Res. 83:586.
  16. Liu, Y.-H. et al. (2008) J. Immunol. 181:6584.
Entrez Gene IDs
972 (Human); 16149 (Mouse); 25599 (Rat)
Alternate Names
CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33

Citations for Recombinant Human CD74 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Targeting CD74 in B-cell non-Hodgkin lymphoma with the antibody-drug conjugate STRO-001
    Authors: X Li, C Abrahams, A Yu, M Embry, R Henningsen, V DeAlmeida, S Matheny, T Kline, A Yam, R Stafford, T Hallam, M Lupher, A Molina
    Oncotarget, 2023-01-12;14(0):1-13.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: SPR
  2. High Frequency of CD74 Expression in Lymphomas: Implications for Targeted Therapy Using Novel Anti-CD74-Drug Conjugate
    Authors: S Zhao, A Molina, A Yu, J Hanson, H Cheung, X Li, Y Natkunam
    J Pathol Clin Res, 2018-10-08;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Reference Standard


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