Home / DDB1/DCAF8 Complex / Recombinant Human DDB1/DCAF8 Complex Protein, CF

Recombinant Human DDB1/DCAF8 Complex Protein, CF

Catalog #: E3-503 Datasheet / COA / SDS

Discontinued Product

E3-503 has been discontinued.
View all DDB1/DCAF8 Complex products.
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human DDB1/DCAF8 Complex Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical concentration to support in vitro applications will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1/DCAF8 Complex protein
Accession #
Q16531, Q5TAQ9
Predicted Molecular Mass
127 kDa (DDB1), 67 kDa (DCAF8)

Product Datasheets

You must select a language.

x

E3-503

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-503

Formulation X mg/ml (X  μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 60 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DDB1/DCAF8 Complex

DDB1- and CUL4-associated factor 8 (DCAF8) is a putative substrate receptor for an E3 Ubiquitin ligase located in the cytoplasm and cell nucleus.  In myocytes, DCAF8 colocalizes with MuRF1 and the two ligases have been shown to contribute to muscle atrophy.  Knockdown of DCAF8 in C2C12 myotubes inhibits myosin heavy chain degradation in a model system for muscle wasting.  In hepatocytes, DCAF8 contributes to progressive ubiquitination of lysine 79 on histone H3, a process that plays an important role in postnatal liver maturation.  This product consists of DDB1 (UniProt Q16531) and DCAF8 (UniProt Q5TAQ9).

References
  1. Jin, J. et al. (2006) Mol. Cell  23: 709
  2. Li, G. et al. (2017) Cell Rep.  18: 1499 doi:10.1016/j.celrep.2017.01.039
  3. Nowak, M. et al. (2019) J. Cell Sci.  132: jsc233395. doi: 10.1242/jcs.233395
Long Name
Damage-specific DNA Binding Protein 1/DDB1 And CUL4 Associated Factor 8 Complex
Alternate Names
DDB1/DCAF8 Complex

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human DDB1/DCAF8 Complex Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human DDB1/DCAF8 Complex Protein, CF and earn rewards!

Have you used Recombinant Human DDB1/DCAF8 Complex Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review