Recombinant Human Dkk-1 Biotinylated Protein

    
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  • Purity
    >95%, by SDS-PAGE with silver staining
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.2-1 μg/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt-3a (Catalog # 1324-WN). 
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dkk-1 protein
    Thr32-His266
    Accession # O94907
  • Accession #
  • N-terminal Sequence
    Analysis
    Thr32
  • Structure / Form
    Monomer, biotinylated via sugars
  • Predicted Molecular Mass
    26 kDa (unlabeled)
  • SDS-PAGE
    33-38 kDa, reducing conditions
Product Datasheets

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Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
BT5439
 
BT5439/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
 
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dkk-1

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains (CRDs) separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 83% amino acid sequence identity with mouse and rat Dkk-1. Dkk-1 is a well-characterized antagonist of Wnt/beta-Catenin signaling (1, 2, 4, 5). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP-5 or LRP-6 (4). Crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6 simultaneously (4, 6-8). Dkk-1/LRP-6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 9). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (10, 11). Other sites of expression include developing neurons, hair follicles, and the retina (12, 13). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (14). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (12, 15). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (16). Dkk-1 also likely has a complex role in cancer, as it has been shown to act as a tumor suppressor and also to promote metastasis (17-19).

  • References:
    1. Krupnik, V.E. et al. (1999) Gene 238:301.
    2. Niehrs, C. (2006) Oncogene 25:7469.
    3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582.
    4. Mao, B. et al. (2001) Nature 411:321.
    5. Brott, B.K. and S.Y. Sokol (2002) Mol. Cell. Biol. 22:6100.
    6. Chen, S. et al. (2011) Dev. Cell 21:848.
    7. Bourhis, E. et al. (2011) Structure 19:1433.
    8. Cheng, Z. et al. (2011) Nat. Struct. Mol. Biol. 18:1204.
    9. Mao, B. et al. (2002) Nature 417:664.
    10. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
    11. Adamska, M. et al. (2004) Dev. Biol. 272:134.
    12. Li, J. et al. (2006) Bone 39:754.
    13. Verani, R. et al. (2007) J. Neurochem. 100:242.
    14. Pinzone, J.J. et al. (2009) Blood 113:517.
    15. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
    16. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.
    17. Menezes, M.E. et al. (2012) Int. J. Cancer 130:1477.
    18. Li, S. et al. (2013) PLoS One 8:e84944.
    19. Chen, L. et al. (2013) Mol. Cancer 12:157.
  • Long Name:
    Dickkopf-1
  • Entrez Gene IDs:
    22943 (Human); 13380 (Mouse)
  • Alternate Names:
    dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like
Related Research Areas

FAQs

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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
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Streptavidin-Phycoerythrin

Flow F0040 7
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NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
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Streptavidin-Allophycocyanin

Flow F0050 1
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NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
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NorthernLights Streptavidin NL637

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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 50  
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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