Recombinant Human FABP1/L-FABP Protein, CF

  
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Bioassay data are not available.
  • Source
    E. coli-derived Ser2-Ile127, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser2
  • Predicted Molecular Mass
    15 kDa
  • SDS-PAGE
    14 kDa, reducing conditions
9465-CL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP1/L-FABP
Fatty acid binding protein-1 (FABP1; also named L- or liver FABP, hepatic FABP, Z protein, and heme-binding protein) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 10, 11). FABP1 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126‑140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (IL-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Human L-FABP, the product of the FABP1 gene, is a 127 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 7). Unlike other members in the FABP family, each molecule of FABP1 is capable of binding two molecules of long-chain fatty acids. Other ligands may include heme, steroids, acyl CoAs, oxidized/peroxidized fatty acids, leukotrienes, prostaglandins and peroxisome proliferators (5, 6, 7, 13). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (7, 12). An Ala-to-Thr polymorphism at position # 54 has been associated with an increased risk of type II diabetes (7, 10) and a Thr-to-Ala polymorphism at position # 94 has been reported to increase cholesterol binding affinity (8). Human FABP1 is 84%, 82% and 90% aa identical to mouse, rat and canine FABP1, respectively (9). It also shows 29% and 24% aa identity to human H-FABP and I‑FABP, respectively.
  • References:
    1. Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
    2. Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
    3. Mihajlovic,M. & Lazaridis, T. (2007) Protein Sci. 9:2042.
    4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
    5. Wang, G. et al. (2015) J Lipid Res. 56:2238.
    6. Thompson, J. et al. (1997) J. Biol. Chem. 272:7140.
    7. Bernlohr, DA. et al. (1997) Ann. Rev. of Nut. 17:277.
    8. Huang, H. et al. (2015) Biochim Biophys Acta. 1851:946
    9. Lowe J.B. et al. (1985) J. Biol. Chem. 260:3413.
    10. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
    11. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
    12. Majava,V. et al. (2010) PLoS One. 5:e10300.
    13. Veerkamp JH, Maatman RGHJ. (1995) Prog. Lipid Res. 34:17.
  • Long Name:
    Fatty Acid-Binding Protein 1
  • Entrez Gene IDs:
    2168 (Human); 14080 (Mouse); 24360 (Rat)
  • Alternate Names:
    FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 26  
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His Tag HRP-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 2  
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His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC050T
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His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC0501V
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His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC0501S
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC0501N
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His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC050R
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His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC050U
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His Tag Alexa Fluor® 594-conjugated Antibody

ICFlow IC0501T
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His Tag Alexa Fluor® 647-conjugated Antibody

ICFlow IC0501R
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His Tag Alexa Fluor® 405-conjugated Antibody

ICFlow IC050V
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His Tag Alexa Fluor® 750-conjugated Antibody

ICFlow IC050S
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His Tag Alexa Fluor® 350-conjugated Antibody

ICFlow IC0501U
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC0501G
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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