Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI8894-050
Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF Binding Activity
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Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5 µg/mL (100 µL/well), Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) binds with an ED50 of 50-300 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein
Human Fc gamma RIIIA (F176)
(Gly17-Gln208)
Accession # P08637-1
HHHHHHHAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Gly17
Structure / Form
Biotinylated protein via Avi-tag
Predicted Molecular Mass
25 kDa
SDS-PAGE
48-57 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI8894

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Human IgG is immobilized at 5 µg/mL, Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) binds with an ED50 of 50-300 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 48-57 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIIA/CD16a

Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 192 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 21 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (V176) and low binding (F176) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # AVI8894 is expressed as the F176 isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).

References
  1. Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19. 
  2. Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481. 
  3. Wu, J. et al. (1997) J. Clin. Invest. 100:1059. 
  4. Dall'Ozzo, S. et al. (2004) Cancer Res. 64:4664. 
  5. Kim, M.-K. et al. (2003) Blood 101:4479. 
  6. Lanier, L.L. et al. (1989) Nature 342:803.
  7. Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
  8. Li, P. et al. (2007) J. Biol. Chem. 282:6210.
  9. Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
  10. Masuda, M. et al. (2006) Atherosclerosis 188:377.
  11. Webster, N.L. et al.(2006) J. Leukoc. Biol. 79:294.
Long Name
Fc gamma Receptor III A
Entrez Gene IDs
2214 (Human); 304966 (Rat)
Alternate Names
CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; Fc gamma RIIIA; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA

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