Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF Summary
Why choose R&D Systems Avi-tag Fc gamma RIIIA Protein?
- Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 95%.
- Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
- Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
- Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.
Find the full range of Avi-tag and amine biotinylated proteins Avi-tag Proteins
Fc gamma RIIIA (F176)|
Accession # P08637-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Human IgG is immobilized at 5 µg/mL, Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) binds with an ED50 of 50-300 ng/mL.
2 μg/lane of Biotinylated Recombinant Human Fc gamma RIIIA/CD16a (F176) His-tag Avi-tag (Catalog # AVI8894) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-57 kDa.
Background: Fc gamma RIIIA/CD16a
Fc gamma RIIIa is a low/intermediate affinity receptor for polyvalent immune-complexed IgG. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes (1, 2). In humans, it is a 50-70 kDa type I transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages (1). Fc gamma RIIIb is highly related, sharing 97% amino acid (aa) identity within the extracellular domain (ECD), but is a GPI-linked receptor expressed on human neutrophils and eosinophils (1, 2). The ECD of Fc gamma RIIIa shares 63%, 61%, 65%, 59% and 58% aa identity with mouse Fc gamma RIV, rat Fc gamma RIIIa, feline CD16, bovine CD16 and porcine Fc gamma RIIIb paralogs, respectively. The Fc gamma RIIIa cDNA encodes 254 aa including a 16 aa signal sequence, 192 aa ECD with two C2-type Ig-like domains and five potential N-glycosylation sites, a 21 aa transmembrane (TM) sequence and a 25 aa cytoplasmic domain. In humans, a single nucleotide polymorphism creates high binding (V176) and low binding (F176) forms that, when homozygous, may influence susceptibility to autoimmune diseases or response to therapeutic IgG antibodies (3, 4). Catalog # AVI8894 is expressed as the F176 isoform of Fc gamma RIIIa. Fc gamma RIIIa surface expression requires interaction of an accessory chain, either the common gamma -chain or CD3 zeta (5, 6). Glycosylation patterns, electrophoretic mobility and binding affinity appear to differ between NK cell and monocyte Fc gamma RIIIa (7). The ECD of both Fc gamma RIIIa and b can be proteolytically cleaved and retain binding activity in soluble form (8-11). In monocytes and macrophages, activation and phagocytosis can trigger Fc gamma RIIIa release (11). Soluble Fc gamma RIII can be detected in normal plasma and is increased in rheumatoid arthritis and in coronary artery diseases (9, 10).
- Nimmerjahn, F. and J.V. Ravetch (2006) Immunity 24:19.
- Ravetch, J.V. and B. Perussia (1989) J. Exp. Med. 170:481.
- Wu, J. et al. (1997) J. Clin. Invest. 100:1059.
- Dall'Ozzo, S. et al. (2004) Cancer Res. 64:4664.
- Kim, M.-K. et al. (2003) Blood 101:4479.
- Lanier, L.L. et al. (1989) Nature 342:803.
- Edberg, J.C. and R.P. Kimberley (1997) J. Immunol. 159:3849.
- Li, P. et al. (2007) J. Biol. Chem. 282:6210.
- Masuda, M. et al. (2003) J. Rheumatol. 30:1911.
- Masuda, M. et al. (2006) Atherosclerosis 188:377.
- Webster, N.L. et al.(2006) J. Leukoc. Biol. 79:294.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Supplemental Cell Selection Products
Supplemental ELISA Products
Reviews for Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF and earn rewards!
Have you used Recombinant Human Fc gamma RIIIA (F176) His Avi Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image