Recombinant Human FGF-4 Protein

A New and Improved rhFGF-4 is Now Available! It has enhanced purity!
New Product 7460-F4.
(29 citations)
(1 Review)
  
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.25-1.25 ng/mL.
  • Source
    E. coli-derived Ser54-Leu206
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser54 & Leu55
  • Predicted Molecular Mass
    17 kDa
  • SDS-PAGE
    16 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
235-F4
 
235-F4/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human FGF-4 (Catalog # 235‑F4) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.25‑1.25 ng/mL.
1 μg/lane of Recombinant Human FGF-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.
Background: FGF-4

FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature human FGF-4 (aa 71-206) shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the mouse trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in mouse development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

  • References:
    1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
    2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
    3. Niswander, L. and G.R. Martin (1992) Development 114:755.
    4. Feldman, B. et al. (1995) Science 267:246.
    5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
    6. Sun, X. et al. (2002) Nature 418:501.
    7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
    8. Yu, K and D.M. Ornitz (2008) Development 135:483.
    9. Mariani, F.V. et al. (2008) Nature 453:401.
    10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
    11. Kunath, T. et al. (2007) Development 134:2895.
    12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
    13. Hajitou, A. et al. (1998) Oncogene 17:2059.
    14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.
  • Long Name:
    Fibroblast Growth Factor 4
  • Entrez Gene IDs:
    2249 (Human); 14175 (Mouse); 116499 (Rat)
  • Alternate Names:
    FGF4; FGF-4; fibroblast growth factor 4; HBGF-4; HBGF-4Transforming protein KS3; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST-1; HST-1HSTF-1; HSTF1fibroblast growth factor 4 splice isoform; HSTFGF-4; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; KFGF; K-FGF; KS3; oncogene HST
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling
    Authors: JO Múnera, N Sundaram, SA Rankin, D Hill, C Watson, M Mahe, JE Vallance, NF Shroyer, KL Sinagoga, A Zarzoso-La, JR Hudson, JC Howell, P Chatuvedi, JR Spence, JM Shannon, AM Zorn, MA Helmrath, JM Wells
    Cell Stem Cell, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Wnt/?-catenin promotes gastric fundus specification in mice and humans
    Authors: KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells
    Nature, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Growth hormone is permissive for neoplastic colon growth
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst
    Nat Commun, 2016;7(0):13463.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  5. Intestinal Commitment and Maturation of Human Pluripotent Stem Cells Is Independent of Exogenous FGF4 and R-spondin1.
    Authors: Tamminen K, Balboa D, Toivonen S, Pakarinen M, Wiener Z, Alitalo K, Otonkoski T
    PLoS ONE, 2015;10(7):e0134551.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Loss of LKB1 leads to impaired epithelial integrity and cell extrusion in the early mouse embryo.
    Authors: Krawchuk D, Anani S, Honma-Yamanaka N, Polito S, Shafik M, Yamanaka Y
    J Cell Sci, 2015;128(5):1011-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Persistence and toxin production by Clostridium difficile within human intestinal organoids result in disruption of epithelial paracellular barrier function.
    Authors: Leslie J, Huang S, Opp J, Nagy M, Kobayashi M, Young V, Spence J
    Infect Immun, 2015;83(1):138-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Identification and characterization of mesenchymal-epithelial progenitor-like cells in normal and injured rat liver.
    Authors: Liu D, Yovchev M, Zhang J, Alfieri A, Tchaikovskaya T, Laconi E, Dabeva M
    Am J Pathol, 2015;185(1):110-28.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  9. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
    Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
    EMBO J, 2015;35(3):356-68.
  10. Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.
    Authors: Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L
    J Virol, 2015;89(9):5134-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. HHEX promotes hepatic-lineage specification through the negative regulation of eomesodermin.
    Authors: Watanabe, Hitoshi, Takayama, Kazuo, Inamura, Mitsuru, Tachibana, Masashi, Mimura, Natsumi, Katayama, Kazufumi, Tashiro, Katsuhis, Nagamoto, Yasuhito, Sakurai, Fuminori, Kawabata, Kenji, Furue, Miho Kus, Mizuguchi, Hiroyuki
    PLoS ONE, 2014;9(3):e90791.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. CCAAT/enhancer binding protein-mediated regulation of TGFbeta receptor 2 expression determines the hepatoblast fate decision.
    Authors: Takayama K, Kawabata K, Nagamoto Y, Inamura M, Ohashi K, Okuno H, Yamaguchi T, Tashiro K, Sakurai F, Hayakawa T, Okano T, Furue M, Mizuguchi H
    Development, 2014;141(1):91-100.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Rapid and efficient differentiation of human pluripotent stem cells into intermediate mesoderm that forms tubules expressing kidney proximal tubular markers.
    Authors: Lam A, Freedman B, Morizane R, Lerou P, Valerius M, Bonventre J
    J Am Soc Nephrol, 2014;25(6):1211-25.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Differentiation-dependent requirement of Tsix long non-coding RNA in imprinted X-chromosome inactivation.
    Authors: Maclary E, Buttigieg E, Hinten M, Gayen S, Harris C, Sarkar M, Purushothaman S, Kalantry S
    Nat Commun, 2014;5(0):4209.
    Species: Mouse
    Sample Type: Embryo
    Application: Bioassay
  15. Long-term self-renewal of human ES/iPS-derived hepatoblast-like cells on human laminin 111-coated dishes.
    Authors: Takayama K, Nagamoto Y, Mimura N, Tashiro K, Sakurai F, Tachibana M, Hayakawa T, Kawabata K, Mizuguchi H
    Stem Cell Reports, 2013;1(4):322-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells.
    Authors: Wang P, McKnight K, Wong D, Rodriguez R, Sugiyama T, Gu X, Ghodasara A, Qu K, Chang H, Kim S
    Stem Cells Dev, 2012;21(12):2273-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Derivation of cerebellar neurons from human pluripotent stem cells.
    Authors: Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S
    Curr Protoc Stem Cell Biol, 2012;20(0):1H.51.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  18. Self-regulation of Stat3 activity coordinates cell-cycle progression and neural crest specification.
    EMBO J., 2010;29(1):55-67.
    Species: Xenopus
    Sample Type: Whole Cells
    Application: Bioassay
  19. Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells.
    Authors: Roelandt P, Pauwelyn KA, Sancho-Bru P
    PLoS ONE, 2010;5(8):e12101.
    Species: Human
    Sample Type: Whole Cells
    Application: Expansion/Differentiation
  20. Altered splicing of FGFR1 is associated with high tumor grade and stage and leads to increased sensitivity to FGF1 in bladder cancer.
    Authors: Tomlinson DC, Knowles MA
    Am. J. Pathol., 2010;177(5):2379-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Heparin-binding secretory transforming gene (hst) facilitates rat lactotrope cell tumorigenesis and induces prolactin gene transcription.
    Authors: Shimon I, Huttner A, Said J, Spirina OM, Melmed S
    J. Clin. Invest., 2009;97(1):187-95.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  22. Fibroblast growth factors and epidermal growth factor cooperate with oocyte-derived members of the TGFbeta superfamily to regulate Spry2 mRNA levels in mouse cumulus cells.
    Authors: Sugiura K, Su YQ, Li Q, Wigglesworth K, Matzuk MM, Eppig JJ
    Biol. Reprod., 2009;81(5):833-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Role for amplification and expression of glypican-5 in rhabdomyosarcoma.
    Authors: Williamson D, Selfe J, Lu YJ, Pritchard-Jones K, Murai K, Jones P, Workman P, Shipley J
    Cancer Res., 2007;67(1):57-65.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: Bioassay
  25. Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements.
    Authors: Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C
    Dev. Biol., 2006;298(2):585-96.
    Species: Chicken
    Sample Type: In Vivo
    Application: In Vivo
  26. FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.
    Authors: Nakayama T, Mutsuga N, Tosato G
    Blood, 2006;109(4):1363-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. An early role for WNT signaling in specifying neural patterns of Cdx and Hox gene expression and motor neuron subtype identity.
    Authors: Nordstrom U, Maier E, Jessell TM, Edlund T
    PLoS Biol., 2006;4(8):e252.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Bioassay
  28. Multipotent adult progenitor cells from swine bone marrow.
    Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C
    Stem Cells, 2006;24(11):2355-66.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  29. FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis.
    Authors: Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP
    Development, 2005;132(6):1223-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 29 Citations
Animal-Free Recombinant Proteins
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Recombinant Human FGF-4, Animal-Free Protein

BA AFL235
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We have 1 review tested in 1 application: Stem/Immune cell maintenance or differentiation .
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 Stem/Immune cell maintenance or differentiation Anonymous 01/19/2016
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Summary

ApplicationStem/Immune cell maintenance or differentiation
Reason for RatingThis growth factor worked perfectly for the maintenance of trophoblast stem cells.

Other Experimental Details

Other Experimental DetailsTrophoblast stem cells are maintained in the presence of growth factors including FGF-4 (cat # 235-F4). When the growth factors are removed, the cells differentiate into giant cells (see image).

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