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Recombinant Human Flag-DDB1His10-/DCAF16 Complex Protein, CF

Catalog #: E3-502 Datasheet / COA / SDS

Discontinued Product

E3-502 has been discontinued.
View all DDB1/DCAF16 Complex products.
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Recombinant Human Flag-DDB1His10-/DCAF16 Complex Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Typical concentration to support in vitro applications will depend on experimental conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1/DCAF16 Complex protein
Accession #
Q16531, Q9NXF7
Predicted Molecular Mass
131 kDa (DDB1), 27 kDa (DCAF16)

Product Datasheets

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E3-502

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-502

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM DTT
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 48 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DDB1/DCAF16 Complex

DDB1- and CUL4-associated factor 16 (DCAF16) is a putative substrate receptor for an E3 Ubiquitin ligase located in the cell nucleus.  A new class of electrophilic ligand-dependent protein degraders that function through DCAF16 has recently been described.  DCAF16 is a component of DCX (DDB1-CUL4-X-box) ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2.  This product consists of a FLAG-tagged DDB1 (UniProt Q16531) and His-tagged DCAF16 (Uniprot Q9NXF7).

References
  1. Jin, J. et al. (2006) Mol. Cell  23: 709
  2. Zhang, X. et al.  (2019) Nat. Chem. Bio.  15: 737
Long Name
Damage-specific DNA Binding Protein 1/DDB1 And CUL4 Associated Factor 16 Complex
Entrez Gene IDs
1642 (Human)
Alternate Names
DDB1/DCAF16 Complex

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