Recombinant Human GDF-5 Protein, CF

(1 citations)   
  • Purity
    >90%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2-1.2 μg/mL.
  • Source
    E. coli-derived Ala382-Arg501
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala382 and Pro383
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    14 kDa
  • SDS-PAGE
    14 kDa, reducing conditions
8340-G5
 
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1.2 μg/mL.
Background: GDF-5/BMP-14
Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).
  • References:
    1. Storm, E.E. et al. (1994) Nature 368:639.
    2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
    3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
    4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
    5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
    6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
    7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
    8. Osorio, C. et al. (2013) Development 140:4751.
    9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
    10. Francis-West, P.H. et al. (1999) Development 126:1305.
    11. Brunet, L.J. et al. (1998) Science 280:1455.
    12. Zeng, Q. et al. (2007) Bone 40:374.
    13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
    14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
    15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
    16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
    17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
    18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
  • Long Name:
    Growth Differentiation Factor 5
  • Entrez Gene IDs:
    8200 (Human); 14563 (Mouse)
  • Alternate Names:
    BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
    Authors: Lorda-Diez C, Montero J, Choe S, Garcia-Porrero J, Hurle J
    J Bone Miner Res, 2014;29(3):735-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay

Have you used Recombinant Human GDF-5 Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

    Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information