Recombinant Human GDF-5 Protein, CF

Catalog # Availability Size / Price Qty
8340-G5-050
Recombinant Human GDF-5 Protein, CF Bioactivity
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Recombinant Human GDF-5 Protein, CF Summary

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2-1.2 μg/mL.
Source
E. coli-derived human GDF-5/BMP-14 protein
Ala382-Arg501
Accession #
N-terminal Sequence
Analysis
Ala382 and Pro383
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
14 kDa
SDS-PAGE
14 kDa, reducing conditions

Product Datasheets

8340-G5

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Human GDF-5 Protein, CF Bioactivity View Larger

Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1.2 μg/mL.

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Background: GDF-5/BMP-14

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
  3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
  4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
  6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
  7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
  8. Osorio, C. et al. (2013) Development 140:4751.
  9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
  10. Francis-West, P.H. et al. (1999) Development 126:1305.
  11. Brunet, L.J. et al. (1998) Science 280:1455.
  12. Zeng, Q. et al. (2007) Bone 40:374.
  13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
  14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
  15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
  16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
  17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
  18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
Long Name
Growth Differentiation Factor 5
Entrez Gene IDs
8200 (Human); 14563 (Mouse)
Alternate Names
BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2

Citation for Recombinant Human GDF-5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
    Authors: Lorda-Diez C, Montero J, Choe S, Garcia-Porrero J, Hurle J
    J Bone Miner Res, 2014;29(3):735-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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