Recombinant Human GDF-5 Protein, CF

Catalog # Availability Size / Price Qty
8340-G5-050
Recombinant Human GDF-5 Protein Bioactivity
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Citations (2)
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Recombinant Human GDF-5 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.2-1.2 μg/mL.
Source
E. coli-derived human GDF-5/BMP-14 protein
Ala382-Arg501
Accession #
N-terminal Sequence
Analysis
Ala382 and Pro383
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
14 kDa
SDS-PAGE
14 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8340-G5

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 250 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Human GDF-5 Protein Bioactivity View Larger

Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1.2 μg/mL.

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Background: GDF-5/BMP-14

Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
  3. Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
  4. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  5. Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
  6. Bragdon, B. et al. (2011) Cell. Signal. 23:609.
  7. Sieber, C. et al. (2006) Biol. Chem. 387:451.
  8. Osorio, C. et al. (2013) Development 140:4751.
  9. Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
  10. Francis-West, P.H. et al. (1999) Development 126:1305.
  11. Brunet, L.J. et al. (1998) Science 280:1455.
  12. Zeng, Q. et al. (2007) Bone 40:374.
  13. Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
  14. Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
  15. Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
  16. Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
  17. Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
  18. Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
Long Name
Growth Differentiation Factor 5
Entrez Gene IDs
8200 (Human); 14563 (Mouse)
Alternate Names
BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2

Citations for Recombinant Human GDF-5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Generation and characterization of human induced pluripotent stem cells (iPSCs) from hand osteoarthritis patient-derived fibroblasts
    Authors: R Castro-Viñ, C Sanjurjo-R, M Piñeiro-Ra, T Hermida-Gó, S Rodríguez-, N Oreiro, J de Toro, I Fuentes, FJ Blanco, S Díaz-Prado
    Sci Rep, 2020;10(1):4272.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
    Authors: Lorda-Diez C, Montero J, Choe S, Garcia-Porrero J, Hurle J
    J Bone Miner Res, 2014;29(3):735-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the half-life of a recombinant or natural protein in culture at 37 °C?

    • R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

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