Recombinant Human GDF-5 Protein, CF Summary
|Formulation||Lyophilized from a 0.2 μm filtered solution in HCl.|
|Reconstitution||Reconstitute at 250 μg/mL in 4 mM HCl.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Human GDF-5 (Catalog # 8340-G5) induces alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1.2 μg/mL.
Growth Differentiation Factor-5 (GDF-5; also called BMP-14 and CDMP-1) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). Human GDF-5, -6, and -7 are a defined subgroup of the BMP family (3). GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) N-terminal pro-region and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (4, 5). Similar to other BMP family proteins, GDF-5 signaling is antagonized by Noggin (6). GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis (7-11). Exogenous GDF-5 has been reported to promote chondrogenesis, osteogenesis, and angiogenesis in mesenchymal stem cells in vivo and in vitro (12-14). Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis (15-18).
- Storm, E.E. et al. (1994) Nature 368:639.
- Hotten, G. et al. (1994) Biochem. Biophys. Res. Commun. 204:646.
- Mikic, B. (2004) Ann. Biomed. Eng. 32:466.
- Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
- Mueller, T.D. and J. Nickel (2012) FEBS Lett. 586:1846.
- Bragdon, B. et al. (2011) Cell. Signal. 23:609.
- Sieber, C. et al. (2006) Biol. Chem. 387:451.
- Osorio, C. et al. (2013) Development 140:4751.
- Wood, T.K. et al. (2005) J. Neurosci. Res. 80:759.
- Francis-West, P.H. et al. (1999) Development 126:1305.
- Brunet, L.J. et al. (1998) Science 280:1455.
- Zeng, Q. et al. (2007) Bone 40:374.
- Coleman, C.M. et al. (2013) Stem Cells Dev. 22:1968.
- Cheng, X. et al. (2012) Cells Tissues Organs 196:56.
- Zhang, Y. et al. (2014) Exp. Mol. Med. 46:e79.
- Miyamoto, Y. et al. (2007) Nat. Genet. 39:529.
- Vaes, R.B. et al. (2009) Ann. Rheum. Dis. 68:1754.
- Degenkolbe, E. et al. (2013) PLoS Genet. 9:e1003846.
Citation for Recombinant Human GDF-5 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Ligand- and stage-dependent divergent functions of BMP signaling in the differentiation of embryonic skeletogenic progenitors in vitro.
Authors: Lorda-Diez C, Montero J, Choe S, Garcia-Porrero J, Hurle J
J Bone Miner Res, 2014;29(3):735-48.
Sample Types: Whole Cells
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Human GDF-5 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human GDF-5 Protein, CF and earn rewards!
Have you used Recombinant Human GDF-5 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image