Recombinant Human GDF-7 Protein, CF

Catalog # Availability Size / Price Qty
8386-G7-050
GDF-7-induced Alkaline Phosphatase Production.
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Recombinant Human GDF-7 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 0.25-1.25 μg/mL.
Source
E. coli-derived human GDF-7/BMP-12 protein
Thr322-Arg450
Accession #
N-terminal Sequence
Analysis
Thr322
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
14 kDa
SDS-PAGE
14 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8386-G7

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 500 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity GDF-7-induced Alkaline Phosphatase Production. View Larger

Recombinant Human GDF-7 stimulates alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.25-1.25 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GDF-7/BMP-12

Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF­7 is synthesized as a large precursor protein that consists of an N-­terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (3, 4). GDF-7 is involved in tendon and ligament formation and repair (5-8). GDF-7 also regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance in the central nervous system (9-12).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem Biophys Res Commun 204:646.
  3. Nishitoh, H. et al. (1996) J Biol Chem 271:21345.
  4. Mueller, T.D. and J. Nickel (2012) FEBS Lett 586:1846.
  5. Fu, S.C. et al. (2003) Life Sci 72:2965.
  6. Gulati, B.R. et al. (2013) Cells Tissues Organs 198:377.
  7. Shen, H. et al. (2013) PLoS One 8:e77613.
  8. Sena, K. et al. (2003) J Dent Res 82:166.
  9. Butler, S.J. and J. Dodd (2003) Neuron 38:389.
  10. Watakabe, A. et al. (2001) J Neurochem 76:1455.
  11. Lou, J. et al. (2001) J Orthop Res 19:1199.
  12. Wang, Q.W. et al. (2005) J Biosci Bioeng 100:418.
Long Name
Growth Differentiation Factor 7
Entrez Gene IDs
151449 (Human); 238057 (Mouse)
Alternate Names
BMP12; BMP-12; GDF7; GDF-7; growth differentiation factor 7; growth/differentiation factor 7

Citations for Recombinant Human GDF-7 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Tenogenic Potential of Equine Fetal Mesenchymal Stem Cells Under The In Vitro Effect of Bone Morphogenetic Protein-12 (BMP-12)
    Authors: R Oliva, I Núñez, MN Segunda, OA Peralta
    Journal of equine veterinary science, 2021;104(0):103681.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Control of glucose metabolism is important in tenogenic differentiation of progenitors derived from human injured tendons
    Authors: S Izumi, S Otsuru, N Adachi, N Akabudike, M Enomoto-Iw
    PLoS ONE, 2019;14(3):e0213912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis
    Authors: M Huang, J Tailor, Q Zhen, AH Gillmor, ML Miller, H Weishaupt, J Chen, T Zheng, EK Nash, LK McHenry, Z An, F Ye, Y Takashima, J Clarke, H Ayetey, FMG Cavalli, B Luu, BS Moriarity, S Ilkhanizad, L Chavez, C Yu, KM Kurian, T Magnaldo, N Sevenet, P Koch, SM Pollard, P Dirks, MP Snyder, DA Largaespad, YJ Cho, JJ Phillips, FJ Swartling, AS Morrissy, M Kool, SM Pfister, MD Taylor, A Smith, WA Weiss
    Cell Stem Cell, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix
    Authors: SP Roth, S Schubert, P Scheibe, C Gro beta, W Brehm, J Burk
    Cell Transplant, 2018;0(0):9636897187922.
    Species: Equine
    Sample Types: Whole Tissue
    Applications: Bioassay

FAQs

  1. What is the half-life of a recombinant or natural protein in culture at 37 °C?

    • R&D Systems has not determined the half-life of recombinant or natural proteins in culture. For cell-based bioassays, the incubation period of the protein with the cells typically ranges from 48 to 96 hours. Assay conditions for each protein will need to be optimized by each researcher.

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Human GDF-7 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human GDF-7 Protein, CF and earn rewards!

Have you used Recombinant Human GDF-7 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review