<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 0.25-1.25 μg/mL.
Recombinant Human GDF-7 stimulates alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.25-1.25 μg/mL.
Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF7 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (3, 4). GDF-7 is involved in tendon and ligament formation and repair (5-8). GDF-7 also regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance in the central nervous system (9-12).
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