Recombinant Human GDNF Protein, CF

(49 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR).
    The specific activity of recombinant human GDNF is approximately 3.1 x 103 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) at 1 µg/mL can bind Recombinant Human GDNF with an apparent K<1 nM.
  • Source
    Mouse myeloma cell line, NS0-derived Arg109-Ile211
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg109
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    11.6 kDa (monomer)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
212-GD/CF
 
212-GD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human GDNF (Catalog # 212‑GD/CF) induces SH-SY5Y human neuroblastoma cell proliferation in the presence of Recombinant Human GFR alpha ‑1 Fc Chimera (Catalog # 714‑GR). The ED50 for this effect is 2‑12 ng/mL. The ED50 for the competitors is more than 85 ng/mL, which is more than 20‑folder weaker.

1 ug/lane of Recombinant Human GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 17 kDa and 34 kDa, respectively.

Background: GDNF
Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

  • Long Name:
    Glial Cell line-derived Growth Factor
  • Entrez Gene IDs:
    2668 (Human); 25453 (Rat)
  • Alternate Names:
    Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

49 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways
    Authors: J Drouin-Oue, S Lau, PL Brattås, D Rylander O, K Pircs, DA Grassi, LM Collins, R Vuono, A Andersson, G Westergren, C Graff, L Minthon, H Toresson, RA Barker, J Jakobsson, M Parmar
    EMBO Mol Med, 2017;9(8):1117-1131.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Isolation and characterization of string-forming female germline stem cells from ovaries of neonatal mice
    Authors: J Liu, D Shang, Y Xiao, P Zhong, H Cheng, R Zhou
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors
    Authors: SK Goparaju, K Kohda, K Ibata, A Soma, Y Nakatake, T Akiyama, S Wakabayash, M Matsushita, M Sakota, H Kimura, M Yuzaki, SB Ko, MS Ko
    Sci Rep, 2017;7(0):42367.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Alpha-synuclein prevents the formation of spherical mitochondria and apoptosis under oxidative stress
    Authors: S Menges, G Minakaki, PM Schaefer, H Meixner, I Prots, U Schlötzer-, K Friedland, B Winner, TF Outeiro, KF Winklhofer, CA von Arnim, W Xiang, J Winkler, J Klucken
    Sci Rep, 2017;7(0):42942.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Sustained synchronized neuronal network activity in a human astrocyte co-culture system
    Sci Rep, 2016;6(0):36529.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells
    Sci Rep, 2016;6(0):31063.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Gene Expression Differences in Peripheral Blood of Parkinson's Disease Patients with Distinct Progression Profiles
    Authors: Raquel Pinho
    PLoS ONE, 2016;11(6):e0157852.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients
    Elife, 2016;5(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. MicroRNA-302 switch to identify and eliminate undifferentiated human pluripotent stem cells
    Sci Rep, 2016;6(0):32532.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Small molecules increase direct neural conversion of human fibroblasts
    Sci Rep, 2016;6(0):38290.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells
    Authors: Z Zhao, Y Ma, Z Chen, Q Liu, Q Li, D Kong, K Yuan, L Hu, T Wang, X Chen, Y Peng, W Jiang, Y Yu, X Liu
    Front Cell Neurosci, 2016;10(0):291.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Microglial AGE-albumin is critical for neuronal death in Parkinson's disease: a possible implication for theranostics
    Int J Nanomedicine, 2015;10(0):281-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells.
    Authors: Merkle F, Maroof A, Wataya T, Sasai Y, Studer L, Eggan K, Schier A
    Development, 2015;142(4):633-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Human iPSC-derived motoneurons harbouring TARDBP or C9ORF72 ALS mutations are dysfunctional despite maintaining viability.
    Authors: Devlin, Anna-Cla, Burr, Karen, Borooah, Shyamang, Foster, Joshua D, Cleary, Elaine M, Geti, Imbisaat, Vallier, Ludovic, Shaw, Christop, Chandran, Siddhart, Miles, Gareth B
    Nat Commun, 2015;6(0):5999.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Noggin Over-Expressing Mouse Embryonic Fibroblasts and MS5 Stromal Cells Enhance Directed Differentiation of Dopaminergic Neurons from Human Embryonic Stem Cells.
    Authors: Lim M, Shin M, Lee S, Minn Y, Hoh J, Cho Y, Kim D, Lee S, Kim C, Park C
    PLoS ONE, 2015;10(9):e0138460.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Dopaminergic control of autophagic-lysosomal function implicates Lmx1b in Parkinson's disease.
    Authors: Laguna A, Schintu N, Nobre A, Alvarsson A, Volakakis N, Jacobsen J, Gomez-Galan M, Sopova E, Joodmardi E, Yoshitake T, Deng Q, Kehr J, Ericson J, Svenningsson P, Shupliakov O, Perlmann T
    Nat Neurosci, 2015;18(6):826-35.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Assessment of TTX-s and TTX-r Action Potential Conduction along Neurites of NGF and GDNF Cultured Porcine DRG Somata.
    Authors: Jonas R, Klusch A, Schmelz M, Petersen M, Carr R
    PLoS ONE, 2015;10(9):e0139107.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  19. The sirtuin-2 inhibitor AK7 is neuroprotective in models of Parkinson's disease but not amyotrophic lateral sclerosis and cerebral ischemia.
    Authors: Chen X, Wales P, Quinti L, Zuo F, Moniot S, Herisson F, Rauf N, Wang H, Silverman R, Ayata C, Maxwell M, Steegborn C, Schwarzschild M, Outeiro T, Kazantsev A
    PLoS ONE, 2015;10(1):e0116919.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. Direct conversion of human fibroblasts into neuronal restricted progenitors.
    Authors: Zou, Qingjian, Yan, Quanmei, Zhong, Juan, Wang, Kepin, Sun, Haitao, Yi, Xiaoling, Lai, Liangxue
    J Biol Chem, 2014;289(8):5250-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Histone hyperacetylation up-regulates protein kinase Cdelta in dopaminergic neurons to induce cell death: relevance to epigenetic mechanisms of neurodegeneration in Parkinson disease.
    Authors: Jin H, Kanthasamy A, Harischandra D, Kondru N, Ghosh A, Panicker N, Anantharam V, Rana A, Kanthasamy A
    J Biol Chem, 2014;289(50):34743-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Highly efficient generation of induced neurons from human fibroblasts that survive transplantation into the adult rat brain.
    Authors: Pereira, Maria, Pfisterer, Ulrich, Rylander, Daniella, Torper, Olof, Lau, Shong, Lundblad, Martin, Grealish, Shane, Parmar, Malin
    Sci Rep, 2014;4(0):6330.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. iPSC-derived forebrain neurons from FXS individuals show defects in initial neurite outgrowth.
    Authors: Doers, Matthew, Musser, Michael, Nichol, Robert, Berndt, Erich R, Baker, Mei, Gomez, Timothy, Zhang, Su-Chun, Abbeduto, Leonard, Bhattacharyya, Anita
    Stem Cells Dev, 2014;23(15):1777-87.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair.
    Authors: Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C
    J Biol Chem, 2014;289(21):15044-51.
    Species: Synthetic
    Sample Type: Complex Sample Type
    Application: Bioassay
  25. Lineage-specific regulation of epigenetic modifier genes in human liver and brain.
    Authors: Weng, Matthias, Natarajan, Karthick, Scholz, Diana, Ivanova, Violeta, Sachinidis, Agapios, Hengstler, Jan G, Waldmann, Tanja, Leist, Marcel
    PLoS ONE, 2014;9(7):e102035.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Widespread sequence variations in VAMP1 across vertebrates suggest a potential selective pressure from botulinum neurotoxins.
    Authors: Peng, Lisheng, Adler, Michael, Demogines, Ann, Borrell, Andrew, Liu, Huisheng, Tao, Liang, Tepp, William, Zhang, Su-Chun, Johnson, Eric A, Sawyer, Sara L, Dong, Min
    PLoS Pathog, 2014;10(7):e1004177.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  27. alpha-Synuclein oligomers impair neuronal microtubule-kinesin interplay.
    Authors: Prots I, Veber V, Brey S, Campioni S, Buder K, Riek R, Bohm K, Winner B
    J Biol Chem, 2013;288(30):21742-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  28. Isolation, characterization and propagation of mitotically active germ cells from adult mouse and human ovaries.
    Authors: Woods D, Tilly J
    Nat Protoc, 2013;8(5):966-88.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  29. MicroRNA-based promotion of human neuronal differentiation and subtype specification.
    Authors: Stappert L, Borghese L, Roese-Koerner B, Weinhold S, Koch P, Terstegge S, Uhrberg M, Wernet P, Brustle O
    PLoS ONE, 2013;8(3):e59011.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  30. A human Dravet syndrome model from patient induced pluripotent stem cells.
    Authors: Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori M, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S
    Mol Brain, 2013;6(0):19.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  31. Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease.
    Authors: Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C
    Mol Ther, 2013;21(12):2169-80.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  32. A small molecule screen in stem-cell-derived motor neurons identifies a kinase inhibitor as a candidate therapeutic for ALS.
    Authors: Yang Y, Gupta S, Kim K, Powers B, Cerqueira A, Wainger B, Ngo H, Rosowski K, Schein P, Ackeifi C, Arvanites A, Davidow L, Woolf C, Rubin L
    Cell Stem Cell, 2013;12(6):713-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  33. MEK/ERK signaling directly and indirectly contributes to the cyclical self-renewal of spermatogonial stem cells.
    Authors: Hasegawa K, Namekawa S, Saga Y
    Stem Cells, 2013;31(11):2517-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Cell Culture Substrate
  34. RET is a potential tumor suppressor gene in colorectal cancer.
    Authors: Luo, Y, Tsuchiya, K D, Il Park, D, Fausel, R, Kanngurn, S, Welcsh, P, Dzieciatkowski, S, Wang, J, Grady, W M
    Oncogene, 2013;32(16):2037-47.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  35. RET PLC-gamma phosphotyrosine binding domain regulates Ca2+ signaling and neocortical neuronal migration.
    Authors: Lundgren TK, Nakahata K, Fritz N, Rebellato P, Zhang S, Uhlen P
    PLoS ONE, 2012;7(2):e31258.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  36. Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
    FASEB J., 2011;25(0):2604-14.
    Species: Rabbit
    Sample Type: Whole Cells
    Application: Bioassay
  37. Soluble Endoglin Specifically Binds Bone Morphogenetic Proteins 9 and 10 via Its Orphan Domain, Inhibits Blood Vessel Formation, and Suppresses Tumor Growth.
    Authors: Castonguay R, Werner ED, Matthews RG, Presman E, Mulivor AW, Solban N, Sako D, Pearsall RS, Underwood KW, Seehra J, Kumar R, Grinberg AV
    J. Biol. Chem., 2011;286(34):30034-46.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Surface Plasmon Resonance
  38. Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
    Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
    Neuropharmacology, 2010;58(7):1114-21.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  39. Sustained intraspinal delivery of neurotrophic factor encapsulated in biodegradable nanoparticles following contusive spinal cord injury.
    Authors: Wang YC, Wu YT, Huang HY, Lin HI, Lo LW, Tzeng SF, Yang CS
    Biomaterials, 2008;29(34):4546-53.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  40. A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi.
    Authors: Lu B, PereiraPerrin M
    Infect. Immun., 2008;76(8):3530-8.
    Species: Bacteria
    Sample Type: Trypanosoma
    Application: ELISA capture
  41. Lineage selection of functional and cryopreservable human embryonic stem cell-derived neurons.
    Authors: Ladewig J, Koch P, Endl E, Meiners B, Opitz T, Couillard-Despres S, Aigner L, Brustle O
    Stem Cells, 2008;26(7):1705-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  42. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage.
    Authors: Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC
    Stem Cells, 2007;25(6):1511-20.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  43. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  44. Endothelin-3 regulates neural crest cell proliferation and differentiation in the hindgut enteric nervous system.
    Authors: Nagy N, Goldstein AM
    Dev. Biol., 2006;293(1):203-17.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Bioassay
  45. Multipotent adult progenitor cells from swine bone marrow.
    Authors: Zeng L, Rahrmann E, Hu Q, Lund T, Sandquist L, Felten M, O'Brien TD, O'Brien TD, Zhang J, Verfaillie C
    Stem Cells, 2006;24(11):2355-66.
    Species: Porcine
    Sample Type: Whole Cells
    Application: Bioassay
  46. Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons.
    Authors: Girard BM, Young BA, Buttolph TR, Locknar SA, White SL, Parsons RL
    Neuroscience, 2006;139(4):1329-41.
    Species: Guinea Pig
    Sample Type: Whole Cells
    Application: Bioassay
  47. Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
    Authors: Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E
    Mol. Cell. Neurosci., 2005;31(2):251-62.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  48. Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
    Authors: Esper RM, Loeb JA
    J. Neurosci., 2004;24(27):6218-27.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Bioassay
  49. Derivation of midbrain dopamine neurons from human embryonic stem cells.
    Authors: Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(34):12543-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 49 Citations
Recombinant Proteins
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Recombinant Human GFR alpha-1 Fc Chimera Protein, CF

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