Recombinant Human GLP-1R His-tag Protein, CF

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Recombinant Human GLP-1R His-tag Protein Binding Activity.
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Product Details

Recombinant Human GLP-1R His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When GLP-1 peptide (Tocris, Catalog # 5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag binds with an ED50 of 5.00-30.0 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein
Ala21-Glu139, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala2 & Arg24
Predicted Molecular Mass
15 kDa & 16 kDa
40-50 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When GLP-1 peptide (5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) binds with an ED50 of 5.00-30.0 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-50 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: GLP-1R

Glucagon-like Peptide 1 Receptor (GLP1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1-3). Activation of GLP1R signaling regulates secretion of insulin from pancreatic beta -cells in a glucose-dependent manner (4). Human GLP1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (1, 3, 5). The mature ECD of human GLP1R shares 87% and 86% amino acid identity with mouse and rat GLP1R, respectively. GLP1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (2, 5, 6). GLP1R agonists, including GLP1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (6, 7). GLP1R is also expressed in the brain where it is involved in the control of appetite (7).

  1. Ma, H. et al. (2020) Cell Research. 30:1140.
  2. Song, G. et al. (2017) Nature. 546:312.
  3. Hollenstein, K. et al. (2013) Trends Pharmacol Sci. 35:12.
  4. Drucker, D.J. et al. (1987) PNAS. 84(10):3434.
  5. Donnelly, D. (2012) Br. J. Pharmacol. 166:27.
  6. Simo, R. and Hernandez, C. (2017) Diabetes. 66:1453.
  7. Holst, J.J. (2004) Expert Opinion on Emerging Drugs. 9:155.
Long Name
Glucagon-like Peptide 1 Receptor
Entrez Gene IDs
2740 (Human); 14652 (Mouse)
Alternate Names
GLP-1 receptor; GLP1R; GLP-1R; GLP-1-R; glucagon-like peptide 1 receptor; MGC138331


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