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Recombinant Human Glypican 4 Fc Chimera Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Human Glypican 4 Fc Chimera is coated at 500 ng/mL (100 μL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived human Glypican 4 protein
    Human Glypican 4
    (Ala23-Ser529)
    Accession # O75487    		 IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
    O75487
  • N-terminal Sequence
    Analysis
    Ala23 & Ser355
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    84 kDa
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Product Datasheet

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SDS
9195-GP
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 200 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
  
Data Image
Bioactivity

Bioactivity Glypican 4 [Unconjugated]
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When Recombinant Human Glypican 4 Fc Chimera (Catalog # 9195-GP) is coated at500 ng/mL 100 μL/well), the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) thatproduces 50% of the optimal binding response is 0.75-4.5 ng/mL.
Background: Glypican 4
Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).
  • References:
    1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
    2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
    3. Ussar, S. et al. (2012) Diabetes 61:2289.
    4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
    5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
    6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
    7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
    8. Strate, I. et al. (2015) Development 142:1767.
  • Entrez Gene IDs:
    2239 (Human); 14735 (Mouse); 317322 (Rat)
  • Alternate Names:
    Glypican 4; glypican proteoglycan 4; glypican-4; GPC4; K-glypican; K-glypicandJ900E8.1 (glypican 4)
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