Recombinant Human Glypican 4 Fc Chimera Protein, CF
Recombinant Human Glypican 4 Fc Chimera Protein, CF Summary
Product Specifications
| Human Glypican 4 (Ala23-Ser529) Accession # O75487 | IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9195-GP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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When Recombinant Human Glypican 4 Fc Chimera (Catalog # 9195-GP) is coated at 500 ng/mL 100 µL/well), the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.
Reconstitution Calculator
Background: Glypican 4
Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).
- Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
- Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
- Ussar, S. et al. (2012) Diabetes 61:2289.
- Fico, A. et al. (2014) J. Neurosci. 34:8318.
- Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
- Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
- Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
- Strate, I. et al. (2015) Development 142:1767.
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