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Recombinant Human GM-CSF (CHO) Biotinylated Protein, CF

Name: Recombinant Human GM-CSF (CHO) Biotinylated Protein, CF
Brand: R&D Systems
SKU: BT7954
Price: 386 USD
Availability: InStock

R&D Systems | Catalog # BT7954

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

CHO

Accession Number

P04141

Structure / Form

Biotinylated via amines

Conjugate

Biotin

Applications

Bioactivity

View all GM-CSF Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GM-CSF protein
Ala18-Glu144

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

Predicted Molecular Mass

14.5 kDa

SDS-PAGE

14-28 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human GM-CSF (Catalog # BT7954) binds Recombinant Human GM-CSF R alpha (Catalog # 706-GR) in the presence of Recombinant Human Common beta Chain His-tag (Catalog # 9960-CB) with an ED₅₀ of 20.0-240 ng/mL.

Scientific Data Images for Recombinant Human GM-CSF (CHO) Biotinylated Protein, CF

Biotinylated Recombinant Human GM‑CSF Protein Binding Activity.

Biotinylated Recombinant Human GM-CSF Protein (Catalog # BT7954) binds Recombinant Human GM-CSF R alpha (706-GR) in the presence of Recombinant Human Common beta Chain His-tag (9960-CB) with an ED₅₀ of 20.0-240 ng/mL.

Biotinylated Recombinant Human GM‑CSF Protein SDS-PAGE.

1 μg/lane of Biotinylated Recombinant Human GM‑CSF Protein (Catalog # BT7954) was resolved with SDS-PAGE under reducing (R) condition and visualized by silver staining, showing bands at 14-28 kDa.

Formulation, Preparation, and Storage

BT7954

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: GM-CSF

GM‑CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte‑macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM‑CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM‑CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM‑CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy‑induced neutropenia, and GM‑CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM‑CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8‑12). Mature human GM‑CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM‑CSF and 54% with mouse GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high‑affinity receptors for IL‑3 and IL‑5 (13, 14). In addition, GM‑CSF binds a naturally occurring soluble form of GM‑CSF R alpha (15). Human GM‑CSF is active on canine and feline cells but not on murine cells (16‑18).

References

Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
Cao, Y. (2007) J. Clin. Invest. 117:2362.
Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
Heuser, M. et al. (2007) Semin. Hematol. 44:148.
Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
Kaushansky, K. et al. (1992) Biochemistry 31:1881.
Diederichs, K. et al. (1991) Science 254:1779.
Cantrell, M.A. et al. (1985) Proc. Natl. Acad. Sci. 82:6250.
Lee, F. et al. (1985) Proc. Natl. Acad. Sci. 82:4360.
Wong, G.G. et al. (1985) Science 228:810.
Onetto-Pothier, N. et al. (1990) Blood 75:59.
Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
Hogge, G.S. et al. (1990) Cancer Gene Ther. 6:26.
Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136.
Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.