Recombinant Human His6-BAP1 Protein, CF
Recombinant Human His6-BAP1 Protein, CF Summary
Product Specifications
Contains an N-terminal 6-His tag
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-345
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: BAP1
Breast Cancer 1 (BRCA1) Associated Protein-1 (BAP1) is a deubiquitinating enzyme that was identified in a screen for proteins that interact with BRCA1 (1). The human protein has a predicted molecular weight of 81 kDa and shares 93% and 92% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. BAP1 has two functional domains, an N-terminal Ubiquitin C-terminal Hydrolase (UCH) domain and a C-terminal BRCA1-interacting domain. The UCH domain contains an active site cysteine residue, Cys91 in humans, which catalyzes the hydrolysis of Ubiquitin isopeptide bonds (1). BAP1 also contains two consensus nuclear localization sequences, NLS1, from aa 656-661, and NLS2, from aa 717-722. NLS2, but not NLS1, is required for the nuclear localization of BAP1 (2). BAP1 forms a ternary complex with Host Cell Factor 1 (HCF-1) and YY1 and regulates the expression of several genes (3). Deubiquitination of HCF-1 by BAP1 was reported to be required for efficient G1/S phase transition, suggesting that BAP1 may be involved in cell cycle regulation (4). Accumulating evidence indicates that BAP1 also acts as a tumor suppressor (5). For instance, inactivation or loss of BAP1 is associated with several types of cancer, including myelodysplastic syndrome, melanoma, renal cell carcinoma, and malignant mesothelioma (6-9). This recombinant protein has an N-terminal His6-tag. Accession # NP_004647.
- Jensen, D.E. et al. (1998) Oncogene 16:1097.
- Ventii, K.H. et al. (2008) Cancer Res. 68:6953.
- Yu, H. et al. (2010) Mol. Cell. Biol. 30:5071.
- Machida, Y.J. et al. (2009) J. Biol. Chem. 284:34179.
- Goldstein, A.M. (2011) Nat. Genet. 43:925.
- Dey, A. et al. (2012) Science 337:1541.
- Harbour, J.W. et al. (2010) Science 330:1410.
- Pena-Llopis, S. et al. (2012) Nat. Genet. 44:751.
- Yoshikawa, Y. et al. (2012) Cancer Sci. 103:868.
Citation for Recombinant Human His6-BAP1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Deubiquitinase processing of a non-natural linkage of ubiquitinated-PTEN
Authors: Iwase, R;Maisonet, IJ;Lee, K;Buhrlage, SJ;Cole, PA;
Bioorganic chemistry
Species: N/A
Sample Types: Recombinant Protein
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Human His6-BAP1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human His6-BAP1 Protein, CF and earn rewards!
Have you used Recombinant Human His6-BAP1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
