Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

Catalog # Availability Size / Price Qty
E-304-050
Product Details
Citations (37)
FAQs
Reviews (2)

Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Ubiquitin-activating Enzyme/UBE1 protein
Contains an N-terminal Met-Ser-Tyr-Tyr and 6-His tag
Accession #
Predicted Molecular Mass
121 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-304

Formulation

0.61 mg/ml (5 µM) in 50 mM HEPES pH 8.0, 50 mM NaCl, 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

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Background: Ubiquitin-activating Enzyme/UBE1

Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa.  It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes (1-3). UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation (4,5). Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond (5-8). The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating  – Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair (3,9,10). Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy (11).  UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions. This protein has an N-terminal His6 tag.

 

References
  1. Handley, P.M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:258.
  2. Nagai, Y. et al. (1995) J. Cell Sci. 108:2145.
  3. Stephen, A.G. et al. (1996) J. Biol. Chem. 271:15608.
  4. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  5. Schulman, B.A. & J.W. Harper (2009) Nat. Rev. Mol. Cell Biol. 10:319.
  6. Haas, A.L. et al. (1982) J. Biol. Chem. 257:2543.
  7. Haas, A.L. & I.A. Rose (1982) J. Biol. Chem. 257:10329.
  8. Pickart, C.M. et al. (1994) J. Biol. Chem. 269:7115.
  9. Nouspikel, T. & P.C. Hanawalt (2006) Proc. Natl. Acad. Sci. USA 103:16188.
  10. Moundry, P. et al. (2012) Cell Cycle 11:1573.
  11. Ramser, J. et al. (2008) Am. J. Hum. Genet. 82:188.
Entrez Gene IDs
7317 (Human); 22201 (Mouse); 314432 (Rat); 100009225 (Rabbit); 853670 (Yeast)
Alternate Names
A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; GXP1; MGC4781; POC20 centriolar protein homolog; POC20; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1A1S9; UBE1X; ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing); Ubiquitin-activating enzyme E1; Ubiquitinactivating Enzyme; Ubiquitin-activating Enzyme; ubiquitin-like modifier activating enzyme 1; ubiquitin-like modifier-activating enzyme 1

Citations for Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. L ARP7 Is a BRCA1�Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis
    Authors: F Zhang, P Yan, H Yu, H Le, Z Li, J Chen, X Liang, S Wang, W Wei, L Liu, Y Zhang, X Ji, A Xie, W Chen, Z Han, WT Pu, S Chen, Y Chen, K Sun, B Ge, B Zhang
    Cell Rep, 2020;32(4):107974.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  2. piRNA-independent function of PIWIL1 as a co-activator for anaphase promoting complex/cyclosome to drive pancreatic cancer metastasis
    Authors: F Li, P Yuan, M Rao, CH Jin, W Tang, YF Rong, YP Hu, F Zhang, T Wei, Q Yin, T Liang, L Wu, J Li, D Li, Y Liu, W Lou, S Zhao, MF Liu
    Nat. Cell Biol., 2020;0(0):.
    Species: Human
    Sample Types: Recombinat Protein
    Applications: Bioassay
  3. The FBXW7-SHOC2-Raptor Axis Controls the Cross-Talks between the RAS-ERK and mTORC1 Signaling Pathways
    Authors: CM Xie, M Tan, XT Lin, D Wu, Y Jiang, Y Tan, H Li, Y Ma, X Xiong, Y Sun
    Cell Rep, 2019;26(11):3037-3050.e4.
    Applications: Bioassay
  4. Competitive ubiquitination activates the tumor suppressor p53
    Authors: X Li, M Guo, L Cai, T Du, Y Liu, HF Ding, H Wang, J Zhang, X Chen, C Yan
    Cell Death Differ., 2019;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
    Authors: M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang
    Nat. Chem. Biol., 2019;15(3):276-284.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay
  6. PCGF5 is required for neural differentiation of embryonic stem cells
    Authors: M Yao, X Zhou, J Zhou, S Gong, G Hu, J Li, K Huang, P Lai, G Shi, AP Hutchins, H Sun, H Wang, H Yao
    Nat Commun, 2018;9(1):1463.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Phosphorylation of Arabidopsis SINA2 by CDKG1 affects its ubiquitin ligase activity
    Authors: Y Chen, M Fokar, M Kang, N Chen, RD Allen, Y Chen
    BMC Plant Biol., 2018;18(1):147.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  8. The E3 ubiquitin ligase HERC1 controls the ERK signaling pathway targeting C-RAF for degradation
    Authors: T Schneider, A Martinez-M, M Cubillos-R, R Bartrons, F Ventura, JL Rosa
    Oncotarget, 2018;9(59):31531-31548.
    Applications: Bioassay
  9. Thalidomide promotes degradation of SALL4, a transcription factor implicated in Duane Radial Ray syndrome
    Authors: KA Donovan, J An, RP Nowak, JC Yuan, EC Fink, BC Berry, BL Ebert, ES Fischer
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  10. RNF126 Quenches RNF168 Function in the DNA Damage Response
    Authors: L Zhang, Z Wang, R Shi, X Zhu, J Zhou, B Peng, X Xu
    Genomics Proteomics Bioinformatics, 2018;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  11. Expression, purification, and characterization of the TRIM49 protein
    Authors: DS Guimaraes, MD Gomes
    Protein Expression and Purification, 2018;143(0):57-61.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  12. Ub-ProT reveals global length and composition of protein ubiquitylation in cells
    Authors: H Tsuchiya, D Burana, F Ohtake, N Arai, A Kaiho, M Komada, K Tanaka, Y Saeki
    Nat Commun, 2018;9(1):524.
    Applications: Bioassay
  13. Inheritance of the Golgi Apparatus and Cytokinesis Are Controlled by Degradation of GBF1
    Authors: R Magliozzi, ZI Carrero, TY Low, L Yuniati, C Valdes-Que, F Kruiswijk, K van Wijk, AJR Heck, CL Jackson, D Guardavacc
    Cell Rep, 2018;23(11):3381-3391.e4.
    Applications: Bioassay
  14. Peptide inhibitors of the anaphase promoting-complex that cause sensitivity to microtubule poison
    Authors: SC Schuyler, YO Wu, HY Chen, YS Ding, CJ Lin, YT Chu, TC Chen, L Liao, WW Tsai, A Huang, LI Wang, TW Liao, JH Jhuo, V Cheng
    PLoS ONE, 2018;13(6):e0198930.
    Applications: Bioassay
  15. Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1
    Authors: WO Tucker, AB Kinghorn, LA Fraser, YW Cheung, JA Tanner
    Int J Mol Sci, 2018;19(3):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  16. A Regulatory Module Controlling Homeostasis of a Plant Immune Kinase
    Authors: J Wang, LE Grubb, J Wang, X Liang, L Li, C Gao, M Ma, F Feng, M Li, L Li, X Zhang, F Yu, Q Xie, S Chen, C Zipfel, J Monaghan, JM Zhou
    Mol. Cell, 2018;0(0):.
    Applications: Bioassay
  17. Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors
    Authors: TR de Moura, S Mozaffari-, CZK Szabó, J Schmitzová, O Dybkov, C Cretu, M Kachala, D Svergun, H Urlaub, R Lührmann, V Pena
    Mol. Cell, 2018;69(6):979-992.e6.
    Species: Bacteria
    Sample Types: Cell Lysates
    Applications: Bioassay
  18. Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants
    Authors: F Wojcik, GP Dann, LY Beh, GT Debelouchi, R Hofmann, TW Muir
    Nat Commun, 2018;9(1):1394.
    Applications: Bioassay
  19. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
    Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
    Mol. Cell, 2017;66(4):503-516.e5.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  20. Activity-Based Probes for HECT E3 ubiquitin ligases
    Authors: R Byrne, T Mund, J Licchesi
    Chembiochem, 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  21. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
    Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
    Mol. Cell, 2017;66(4):503-516.e5.
    Applications: Bioassay
  22. Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry
    Authors: K Kliza, C Taumer, I Pinzuti, M Franz-Wach, S Kunzelmann, B Stieglitz, B Macek, K Husnjak
    Nat. Methods, 2017;0(0):.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  23. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage
    Authors: A Paul, B Wang
    Mol. Cell, 2017;66(4):458-472.e5.
    Applications: Bioassay
  24. Ubiquitinated proteins promote the association of proteasomes with the deubiquitinating enzyme Usp14 and the ubiquitin ligase Ube3c
    Authors: CL Kuo, AL Goldberg
    Proc. Natl. Acad. Sci. U.S.A., 2017;114(17):E3404-E3413.
    Species: Bacteria - E. Coli
    Sample Types: Protein
    Applications: Bioassay
  25. Structure-guided mutagenesis reveals a hierarchical mechanism of Parkin activation
    Authors: MY Tang, M Vranas, AI Krahn, S Pundlik, JF Trempe, EA Fon
    Nat Commun, 2017;8(0):14697.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  26. The adenovirus E4-ORF3 protein functions as a SUMO E3 ligase for TIF-1? sumoylation and poly-SUMO chain elongation
    Proc Natl Acad Sci USA, 2016;113(24):6725-30.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  27. FBXW7 Facilitates Nonhomologous End-Joining via K63-Linked Polyubiquitylation of XRCC4
    Authors: Q Zhang, D Karnak, M Tan, TS Lawrence, MA Morgan, Y Sun
    Mol. Cell, 2016;61(3):419-33.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  28. USP7 is a SUMO deubiquitinase essential for DNA replication
    Authors: E Lecona, S Rodriguez-, J Specks, AJ Lopez-Cont, I Ruppen, M Murga, J Muñoz, J Mendez, O Fernandez-
    Nat Struct Mol Biol, 2016;23(4):270-7.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  29. Hemi-methylated DNA regulates DNA methylation inheritance through allosteric activation of H3 ubiquitylation by UHRF1
    Elife, 2016;5(0):.
    Species: Human
    Sample Types: Protein
    Applications: Ubiquitination
  30. Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal
    Nat Commun, 2016;7(0):12326.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  31. Ubiquilins Chaperone and Triage Mitochondrial Membrane Proteins for Degradation
    Mol Cell, 2016;63(1):21-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. PTP1B controls non-mitochondrial oxygen consumption by regulating RNF213 to promote tumour survival during hypoxia
    Authors: RS Banh, C Iorio, R Marcotte, Y Xu, D Cojocari, AA Rahman, J Pawling, W Zhang, A Sinha, CM Rose, M Isasa, S Zhang, R Wu, C Virtanen, T Hitomi, T Habu, SS Sidhu, A Koizumi, SE Wilkins, T Kislinger, SP Gygi, CJ Schofield, JW Dennis, BG Wouters, BG Neel
    Nat. Cell Biol, 2016;18(7):803-13.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Ubiquitination
  33. ASH2L regulates ubiquitylation signaling to MLL: trans-regulation of H3 K4 methylation in higher eukaryotes.
    Authors: Wu L, Lee S, Zhou B, Nguyen U, Muir T, Tan S, Dou Y
    Mol Cell, 2013;49(6):1108-20.
  34. The F-box protein FBXO44 mediates BRCA1 ubiquitination and degradation.
    Authors: Lu, Yunzhe, Li, Jiezhi, Cheng, Dongmei, Parameswaran, Balaji, Zhang, Shaohua, Jiang, Zefei, Yew, P Renee, Peng, Junmin, Ye, Qinong, Hu, Yanfen
    J Biol Chem, 2012;287(49):41014-22.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  35. The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology, and the metabolome of fibroblasts.
    Authors: Janzer, Andreas, Stamm, Katrin, Becker, Astrid, Zimmer, Andreas, Buettner, Reinhard, Kirfel, Jutta
    J Biol Chem, 2012;287(37):30984-92.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  36. Ubiquitination of neuronal nitric-oxide synthase in the calmodulin-binding site triggers proteasomal degradation of the protein.
    Authors: Clapp, Kelly M, Peng, Hwei-Min, Jenkins, Gary J, Ford, Michael, Morishima, Yoshihir, Lau, Miranda, Osawa, Yoichi
    J Biol Chem, 2012;287(51):42601-10.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  37. p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO.
    Authors: Shibata Y, Oyama M, Kozuka-Hata H, Han X, Tanaka Y, Gohda J, Inoue J
    Nat Commun, 2012;3(0):1061.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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Reviews for Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF

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Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
By Anonymous on 11/06/2018
Application: E1 E2 E3 Autoubiquitination Assay

Recombinant Human His6-Ubiquitin E1 Enzyme (UBE1), CF
By Anonymous on 09/18/2018
Application: Ubiquitination Assay with E1/E2/E3