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Recombinant Human His6-Ubiquitin Protein, CF

Catalog #: U-530
20 Citations Datasheet / COA / SDS
 

Discontinued Product

U-530 has been discontinued.
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Citations (20)
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Recombinant Human His6-Ubiquitin Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human His6-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ubiquitin concentration of 0.2-1 mM.
Source
E. coli-derived human Ubiquitin protein
Contains an N-terminal 6-His tag
Accession #
P0CG47
Predicted Molecular Mass
9.6 kDa

Product Datasheets

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U-530

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-530

Formulation

2.4 mg/ml (250 μM) in 10 mM HEPES pH 7.5
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 24 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).

Functional N-terminal His-tagged Ubiquitin which allows for metal chelate affinity purification of ubiquitinated proteins.

References
  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
Entrez Gene IDs
7314 (Human); 298693 (Rat)
Alternate Names
RPS27A; UBA52; UBB ubiquitin B; UBB; UBC; Ubiquitin

Citations for Recombinant Human His6-Ubiquitin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. The differential interactomes of the KRAS splice variants identify BIRC6 as a ubiquitin ligase for KRAS4A
    Authors: Kochen Rossi, J;Nuevo-Tapioles, C;O'Keefe, RA;Hunkeler, M;Schmoker, AM;Fissore-O'Leary, M;Su, W;Ahearn, IM;Branco, C;Cheong, H;Esposito, D;Clotea, I;Ueberheide, B;Fischer, ES;Philips, MR;
    Cell reports
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. The RING-finger ubiquitin E3 ligase TaPIR1 targets TaHRP1 for degradation to suppress chloroplast function
    Authors: Zhang, R;Wu, Y;Qu, X;Yang, W;Wu, Q;Huang, L;Jiang, Q;Ma, J;Zhang, Y;Qi, P;Chen, G;Jiang, Y;Zheng, Y;Wang, X;Wei, Y;Xu, Q;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  3. Substitution of PINK1 Gly411 modulates substrate receptivity and turnover
    Authors: FC Fiesel, D Fri?ová, CS Hayes, MA Coban, R Hudec, JM Bredenberg, BJ Broadway, BN Markham, T Yan, PK Boneski, G Fiorino, JO Watzlawik, X Hou, AM McCarty, LJ Lewis-Tuff, J Zhong, BJ Madden, A Ordureau, H An, A Puschmann, ZK Wszolek, OA Ross, JW Harper, TR Caulfield, W Springer
    Autophagy, 2022-12-05;0(0):1-22.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  4. The p97 segregase cofactor Ubxn7 facilitates replisome disassembly during S-phase
    Authors: Z Tarcan, D Poovathumk, A Skagia, A Gambus
    The Journal of Biological Chemistry, 2022-07-04;298(8):102234.
    Species: Human
    Sample Types: Recombinant Proteins
  5. ARMC5 is part of an RPB1-specific ubiquitin ligase implicated in adrenal hyperplasia
    Authors: L Lao, I Bourdeau, L Gagliardi, X He, W Shi, B Hao, M Tan, Y Hu, J Peng, B Coulombe, DJ Torpy, HS Scott, A Lacroix, H Luo, J Wu
    Nucleic Acids Research, 2022-06-24;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease
    Authors: SK Pirooznia, H Wang, N Panicker, M Kumar, S Neifert, MA Dar, E Lau, BG Kang, J Redding-Oc, JC Troncoso, VL Dawson, TM Dawson
    Science Advances, 2022-04-01;8(13):eabh1824.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  7. Substitution of PINK1 Gly411 modulates substrate receptivity and turnover
    Authors: FC Fiesel, D Fri?ová, CS Hayes, MA Coban, R Hudec, JM Bredenberg, BJ Broadway, BN Markham, T Yan, PK Boneski, G Fiorino, JO Watzlawik, X Hou, AM McCarty, LJ Lewis-Tuff, J Zhong, BJ Madden, A Ordureau, H An, A Puschmann, ZK Wszolek, OA Ross, JW Harper, TR Caulfield, W Springer
    Autophagy, 2022;0(0):1-22.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  8. Structure of the FA core ubiquitin ligase closing the ID clamp on DNA
    Authors: S Wang, R Wang, C Peralta, A Yaseen, NP Pavletich
    Nature structural & molecular biology, 2021-03-08;28(3):300-309.
    Species: Human, N/A
    Sample Types: Protein, Whole Cells
    Applications: Ubiquitination
  9. Mutual regulation between OGT and XIAP to control colon cancer cell growth and invasion
    Authors: HG Seo, HB Kim, JY Yoon, TH Kweon, YS Park, J Kang, J Jung, S Son, EC Yi, TH Lee, WH Yang, JW Cho
    Cell Death Dis, 2020-09-29;11(9):815.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
    Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
    Cell Rep, 2019-07-09;28(2):541-553.e4.
    Applications: Bioassay
  11. Mechanism for recycling tRNAs on stalled ribosomes
    Authors: MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao
    Nat. Struct. Mol. Biol., 2019-04-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination
  12. TRAIP is a master regulator of DNA interstrand crosslink repair
    Authors: RA Wu, DR Semlow, AN Kamimae-La, OV Kochenova, G Chistol, MR Hodskinson, R Amunugama, JL Sparks, M Wang, L Deng, CA Mimoso, E Low, KJ Patel, JC Walter
    Nature, 2019-03-06;567(7747):267-272.
    Applications: Bioassay
  13. Selection and Characterization of a DNA Aptamer Specifically Targeting Human HECT Ubiquitin Ligase WWP1
    Authors: WO Tucker, AB Kinghorn, LA Fraser, YW Cheung, JA Tanner
    Int J Mol Sci, 2018-03-07;19(3):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  14. Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9
    Authors: CS Yang, K Jividen, A Spencer, N Dworak, L Ni, LT Oostdyk, M Chatterjee, B Ku?mider, B Reon, M Parlak, V Gorbunova, T Abbas, E Jeffery, NE Sherman, BM Paschal
    Mol. Cell, 2017-05-18;66(4):503-516.e5.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  15. Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster
    Authors: S Schunter, R Villa, V Flynn, JB Heidelberg, AK Classen, P Beli, PB Becker
    PLoS ONE, 2017-05-16;12(5):e0177408.
    Species: Worm - Spodoptera frugiperda (Fall Army Worm)
    Sample Types: Protein
    Applications: Bioassay
  16. Ubiquitination and dynactin regulate TMEPAI lysosomal trafficking
    Authors: S Luo, L Jing, T Zhao, Y Li, Z Liu, A Diao
    Sci Rep, 2017-02-20;7(0):42668.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  17. Fine-tuning of ULK1 mRNA and protein levels is required for autophagy oscillation
    Authors: Francesca Nazio
    J. Cell Biol, 2016-12-08;215(6):841-856.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  18. p97 Promotes a Conserved Mechanism of Helicase Unloading During DNA Crosslink Repair
    Mol Cell Biol, 2016-11-14;0(0):.
    Species: Xenopus
    Sample Types: Cell Lysates
    Applications: Binding Assay
  19. Cytosolic quality control of mislocalized proteins requires RNF126 recruitment to Bag6.
    Authors: Rodrigo-Brenni M, Gutierrez E, Hegde R
    Mol Cell, 2014-06-26;55(2):227-37.
    Applications: Bioassay
  20. A novel role for inhibitor of apoptosis (IAP) proteins as regulators of endothelial barrier function by mediating RhoA activation.
    Authors: Hornburger M, Mayer B, Leonhardt S, Willer E, Zahler S, Beyerle A, Rajalingam K, Vollmar A, Furst R
    FASEB J, 2013-12-26;28(4):1938-46.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Ubiquitination

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