Recombinant Human His6-UBP43/USP18, CF
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-600
| Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
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Background: USP18
UBP43, also known as Ubiquitin Specific Peptidase 18 (USP18), is a member of the peptidase C19 family of deubiquitinating enzymes (1). It is a nuclear protein highly expressed in the liver, thymus, and peritoneal macrophages, as well as in monocyte-derived cell lines (1,2). UBP43/USP18 is 372 amino acids (aa) in length with a predicted molecular weight of 43 kDa (1,2). Human UBP43/USP18 shares 72% and 73% aa sequence identity with the mouse and rat orthologs, respectively. UBP43/USP18 is an Interferon-stimulated Gene 15 (ISG15)-specific protease (3). ISG15 is a Ubiquitin-like modifier whose expression is strongly induced in response to type I Interferons (IFNs) and lipopolysaccharide treatment (4). UBP43/USP18 removes ISG15 from ISGylated proteins and thus regulates the levels of IGS15-conjugated proteins in cells (3). UBP43/USP18 is believed to have a role in innate immune responses to infections by pathogenic organisms. It has been shown that mice deficient in UBP43/USP18 are resistant to viral infections (5). UBP43/USP18 also negatively regulates type I IFN signaling by binding to the IFNAR2 subunit of the type I IFN receptor and blocking its association with JAK (6). UBP43/USP18 is also thought to be involved in cancer cell survival (7-10). This recombinant human protein contains an N-terminal His6-tag. Accession # NP_059110.
- Liu, L.Q. et al. (1999) Mol. Cell. Biol. 19:3029.
- Schwer, H. et al. (2000) Genomics 65:44.
- Malakhov, M.P. et al. (2002) J. Biol. Chem. 277:9976.
- Zhang, D. & D.E. Zhang (2011) J. Interferon Cytokine Res. 31:119.
- Ritchie, K.J. et al. (2004) Nat. Med. 10:1374.
- Malakhova, O.A. et al. (2006) EMBO J. 25:2358.
- Feng, Q. et al. (2008) Mol. Cancer Ther. 7:3780.
- Guo, Y. et al. (2010) Cancer Res. 70:9875.
- Duex, J.E. et al. (2011) J. Biol. Chem. 286:25377.
- Guo, Y. et al. (2012) Mol. Cancer Ther. 11:1968.
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