Recombinant Human His6-USP8 Protein, CF

Catalog # Availability Size / Price Qty
E-520-025
Product Details
Citations (2)
FAQs
Reviews (1)

Recombinant Human His6-USP8 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-USP8 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-USP8 concentration of 10-50 nM.
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human USP8 protein
Contains an N-terminal 6-His tag
Accession #
Predicted Molecular Mass
131 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-520

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: USP8

Ubiquitin Specific Peptidase 8 (USP8), also known as Ubiquitin Isopeptidase Y (UBPY), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family. It has a predicted molecular weight of 127.5 kDa (1). Human USP8 is 1118 amino acids (aa) in length and shares 84% aa sequence identity with the mouse and rat orthologs (2). It contains an N-terminal MIT domain (aa 33-116) that mediates endosomal localization, CHMP-binding, and maintenance of ESCRT-0 (3). USP8 also has a rhodanese domain (aa 181-319) that binds NRDP1 Ubiquitin ligase (E3), a SH3 domain binding sequence (aa 405-413), and a C-terminal catalytic domain (aa 734-1110) (2,4). USP8 is a growth-regulated enzyme that controls the internalization and endocytic trafficking of cell surface receptors (1,5). Some receptors are targeted for internalization and degradation by ubiquitination. USP8 has been shown to disrupt the down-regulation of multiple receptors, including EGF R/ErbB1, ErbB2/Her2, and Smoothened, via their deubiquitination (6-9). Conversely, USP8 appears to have the opposite effect on the trafficking of CXCR4, PAR2, and the δ-opioid receptor (10-12). Depletion or catalytic inactivation of USP8 stabilized their expression (10-12). It is thought that deubiquitination of these receptors down-stream of the sorting endosome commits them to lysosomal degradation (10). USP8 can be phosphorylated at Ser680 allowing for 14-3-3-epsilon binding, which subsequently inhibits USP8 activity (13). Additionally, USP8 undergoes tyrosine phosphorylation at its N-terminus following EGF activation of the EGF R/ErbB1 / ErbB2/Her2 receptor complex (7). This recombinant human protein contains an N-terminal His6-tag.

References
  1. Naviglio, S et al. (1998) EMBO J. 17:3241.
  2. Avvakumov, G.V. et al. (2006) J. Biol. Chem. 281:38061.
  3. Row, P.E. et al. (2007) J. Biol. Chem. 282:30929.
  4. Kato, M. et al. (2000) J. Biol. Chem. 275:37481.
  5. Wright, M.H. et al. (2011) Cell Biochem. Biophys. 60:39.
  6. Mizuno, E. et al. (2005) Mol. Biol. Cell 16:5163.
  7. Meijer, I. M. and J.E. van Leeuwen (2011) Cell. Signal. 23:458.
  8. Xia, R. et al. (2012) PLoS Biol. 10:e1001238.
  9. Li, E. et al. (2012) PLoS Biol. 10:e1001239.
  10. Hislop, J.N. et al. (2009) J. Biol. Chem. 284:19361.
  11. Hasdemir, B. et al. (2009) J. Biol. Chem. 284:28453.
  12. Berlin, I. et al. (2010) J. Biol. Chem. 285:37895.
  13. Mizuno, E. et al. (2007) Exp. Cell Res. 313:3624
Long Name
Ubiquitin Specific Protease 8
Entrez Gene IDs
9101 (Human); 84092 (Mouse); 296121 (Rat)
Alternate Names
Deubiquitinating enzyme 8; EC 3.1.2.15; EC 3.4.19.12; HumORF8; KIAA0055hUBPy; MGC129718; ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin isopeptidase Y; ubiquitin specific peptidase 8; ubiquitin specific protease 8; ubiquitin thioesterase 8; Ubiquitin thiolesterase 8; Ubiquitin-specific-processing protease 8; UBPY; UBPYFLJ34456; USP8

Citations for Recombinant Human His6-USP8 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. DNA damage shifts circadian clock time via Hausp-dependent Cry1 stabilization.
    Authors: Papp, Stephani, Huber, Anne-Lau, Jordan, Sabine D, Kriebs, Anna, Nguyen, Madelena, Moresco, James J, Yates, John R, Lamia, Katja A
    Elife, 2015;4(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.
    Authors: Liang, Qin, Dexheimer, Thomas S, Zhang, Ping, Rosenthal, Andrew S, Villamil, Mark A, You, Changjun, Zhang, Qiuting, Chen, Junjun, Ott, Christin, Sun, Hongmao, Luci, Diane K, Yuan, Bifeng, Simeonov, Anton, Jadhav, Ajit, Xiao, Hui, Wang, Yinsheng, Maloney, David J, Zhuang, Zhihao
    Nat Chem Biol, 2014;10(4):298-304.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay

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Recombinant Human His6-USP8 Protein, CF
By Anonymous on 07/02/2019
Application: Enzymatic activity in vitro