Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. Human HSP70/HSPA1A shares 95% and 97% aa sequence identity with the mouse and rat orthologs, respectively. It has an N-terminal nucleotide-binding domain, which contains ATPase activity, and a C-terminal substrate-binding domain (1). HSP70/HSPA1A promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins (2). However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). HSP70/HSPA1A can be regulated post-translationally via multiple mechanisms, including phosphorylation, ubiquitination, and methylation (5-8). For example, unmethylated HSP70/HSPA1A localizes to the cytoplasm, but following methylation on Lys561 it is found only in the nucleus (8). Pathologically, HSP70/HSPA1A has been implicated in the promotion of multiple cancer types (8-10). Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12).
Recombinant Human HSP70/HSPA1A Protein, CF
R&D Systems | Catalog # AP-100
Key Product Details
- R&D Systems E. coli-derived Recombinant Human HSP70/HSPA1A Protein (AP-100)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Met1 - Asp641
Predicted Molecular Mass
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial HSP70/HSPA1A concentration of 2-3 μM for in vitro use. IMPORTANT: HSP40/DNAJB1 (Catalog # AP-110), or another suitable co-chaperone, is required for HSP70/HSPA1A activity and should be used at a concentration that is equimolar to HSP70/HSPA1A.
Reviewed Applications
Read 3 reviews rated 5 using AP-100 in the following applications:
Formulation, Preparation, and Storage
AP-100
| Formulation | Supplied as a solution in HEPES, NaCl, DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: HSP70/HSPA1A
References
- Qi, R. et al. (2013) Nat. Struct. Mol. Biol. 20:900.
- Kampinga, H.H. & E.A. Craig (2010) Nat. Rev. Mol. Cell Biol. 11:579.
- McDonough, H. & C. Patterson (2003) Cell Stress Chaperones 8:303.
- Donnelly, B.F. et al. (2013) J. Biol. Chem. 288:13124.
- Muller, P. et al. (2012) Oncogene [Epub ahead of print].
- Liu, M. et al. (2008) J. Biol. Chem. 283:35783.
- Moore, D.J. et al. (2008) J. Neurochem. 105:1806.
- Cho, H.S. et al. (2012) Nat. Commun. 3:1072.
- Wu, F.H. et al. (2012) Cancer Lett. 317:157.
- Gaudio, E. et al. (2013) Blood 121:351.
- Miyata, Y. et al. (2011) Future Med. Chem. 3:1523.
- Wang, A.M. et al. (2013) Nat. Chem. Biol. 9:112.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional HSP70/HSPA1A Products
Product Documents for Recombinant Human HSP70/HSPA1A Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human HSP70/HSPA1A Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human HSP70/HSPA1A Protein, CF
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Customer Reviews for Recombinant Human HSP70/HSPA1A Protein, CF (3)
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 01/13/2026
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Application: Enzymatic activity in vitroVerified Customer | Posted 11/07/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 08/17/2018
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