Recombinant Human HSP70/HSPA1A Protein, CF

Catalog # Availability Size / Price Qty
AP-100-050
AP-100-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (2)
FAQs
Reviews (2)

Recombinant Human HSP70/HSPA1A Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

HSP70/HSPA1A is a molecular chaperone that assists in the folding of nascent polypeptides and the refolding of denatured proteins, but can also promote their degradation in conjunction with specific E3 ligases, such as CHIP, if either of these processes proceeds inefficiently. Reaction conditions will need to be optimized for each specific application. We recommend an initial HSP70/HSPA1A concentration of 2-3 μM for in vitro use. IMPORTANT: HSP40/DNAJB1 (Catalog # AP-110), or another suitable co-chaperone, is required for HSP70/HSPA1A activity and should be used at a concentration that is equimolar to HSP70/HSPA1A.

Source
E. coli-derived human HSP70/HSPA1A protein
Accession #
Predicted Molecular Mass
70 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AP-100

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8, 100 mM NaCl, 5 mM DTT

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP70/HSPA1A

Heat Shock 70kDa Protein (HSP70), also known as Heat Shock 70kDa Protein 1A (HSPA1A), is a 641 amino acid (aa) member of the HSP70 family of molecular chaperones with a predicted molecular weight of 70 kDa. Human HSP70/HSPA1A shares 95% and 97% aa sequence identity with the mouse and rat orthologs, respectively. It has an N-terminal nucleotide-binding domain, which contains ATPase activity, and a C-terminal substrate-binding domain (1). HSP70/HSPA1A promotes the proper folding of nascent polypeptides and assists in the refolding of denatured proteins (2). However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). HSP70/HSPA1A can be regulated post-translationally via multiple mechanisms, including phosphorylation, ubiquitination, and methylation (5-8). For example, unmethylated HSP70/HSPA1A localizes to the cytoplasm, but following methylation on Lys561 it is found only in the nucleus (8). Pathologically, HSP70/HSPA1A has been implicated in the promotion of multiple cancer types (8-10). Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12).

References
  1. Qi, R. et al. (2013) Nat. Struct. Mol. Biol. [Epub ahead of print].
  2. Kampinga, H.H. & E.A. Craig (2010) Nat. Rev. Mol. Cell Biol. 11:579.
  3. McDonough, H. & C. Patterson (2003) Cell Stress Chaperones 8:303.
  4. Donnelly, B.F. et al. (2013) J. Biol. Chem. 288:13124.
  5. Muller, P. et al. (2012) Oncogene [Epub ahead of print].
  6. Liu, M. et al. (2008) J. Biol. Chem. 283:35783.
  7. Moore, D.J. et al. (2008) J. Neurochem. 105:1806.
  8. Cho, H.S. et al. (2012) Nat. Commun. 3:1072.
  9. Wu, F.H. et al. (2012) Cancer Lett. 317:157.
  10. Gaudio, E. et al. (2013) Blood 121:351.
  11. Miyata, Y. et al. (2011) Future Med. Chem. 3:1523.
  12. Wang, A.M. et al. (2013) Nat. Chem. Biol. 9:112.
Long Name
Heat Shock Protein 70
Entrez Gene IDs
3303 (Human); 15511 (Mouse); 24472 (Rat)
Alternate Names
dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B

Citations for Recombinant Human HSP70/HSPA1A Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Role of macrophage-mediated Toll-like receptor 4-interleukin-1R signaling in ectopic tongue pain associated with tooth pulp inflammation
    Authors: K Kanno, K Shimizu, M Shinoda, M Hayashi, O Takeichi, K Iwata
    J Neuroinflammation, 2020;17(1):312.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  2. Toll-like receptor 4 signaling in trigeminal ganglion neurons contributes tongue-referred pain associated with tooth pulp inflammation.
    Authors: Ohara, Kinuyo, Shimizu, Kohei, Matsuura, Shingo, Ogiso, Bunnai, Omagari, Daisuke, Asano, Masatake, Tsuboi, Yoshiyuk, Shinoda, Masamich, Iwata, Koichi
    J Neuroinflammation, 2013;10(0):139.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Recombinant Human HSP70/HSPA1A Protein, CF

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Recombinant Human HSP70/HSPA1A Protein, CF
By Anonymous on 11/07/2018
Application: Enzymatic activity in vitro

Recombinant Human HSP70/HSPA1A Protein, CF
By Anonymous on 08/17/2018
Application: In vitro bioactivity in cell culture