Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF
Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF Summary
Accession # Q92956.3
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human BTLA (9235-BT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein (Catalog # 11177-HV) binds with an ED50 of 50.0-600 ng/mL.
2 μg/lane of Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein (Catalog # 11177-HV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.
Herpesvirus entry mediator (HVEM), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily. Originally identified as important for entry of herpes simplex virus (HSV) through recognition of HSV glycoprotein D (gD), HVEM has been shown to be involved in the regulation of T cell activity (1). Mature human HVEM consists of an extracellular domain (ECD) with four cysteine-rich domains (CRD), a transmembrane segment, and a cytoplasmic region with a TRAF interaction domain (2). The ECD of human HVEM shares 51% amino acid sequence identity with mouse HVEM. HVEM is found on the membrane of various cell types, including hematopoietic and non-hematopoietic cells, with higher expression in the lung, kidney, and liver (3). HVEM is a receptor for the TNF ligand LIGHT, Ig superfamily members BTLA and CD160, as well as Lymphotoxin-alpha and is involved in bidirectional signaling as both a signaling receptor and a ligand for inhibitory receptors (1,4). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (5,6). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (3,6,7). HVEM enhances the development of CD8+ T cell memory and Treg function (8, 9). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epithelial integrity and host defense (10). HVEM is being investigated as an immune checkpoint inhibitor as it is commonly mutated in lymphomas, while upregulation leads to disease progression and is associated with poor prognosis (11, 12).
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- Liu, W. et al. (2021) J Exp Med. 218:e20211112).
- Demerlé C. et al. (2021) Front Oncol. 2021 11:682007.
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- Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
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- Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
- Shui, J.W. et al. (2012) Nature 488:222.
- Aubert, N. et al. (2021) Cancers (Basel) 13:3009.
- Boice, M. et al. (2016) Cell. 167:405.
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