Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

R&D Systems | Catalog # BT169

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human ICOS Fc Chimera Biotinylated Protein (BT169)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9Y6W8

Structure / Form

Disulfide-linked homodimer

Conjugate

Biotin

Applications

Bioactivity
View all ICOS Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human ICOS protein
Human ICOS
(Glu21-Phe141)
Accession # Q9Y6W8		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

40 kDa (unlabeled)

SDS-PAGE


Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant Human ICOS Fc Chimera binds with an ED50 of 1-6 ng/mL.

Scientific Data Images for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

Recombinant Human ICOS Fc Chimera Biotinylated Protein Binding Activity

Recombinant Human ICOS Fc Chimera Biotinylated Protein Binding Activity

When Recombinant Human B7-H2 Fc Chimera Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) is immobilized at 0.1 µg/mL (100 µL/well), Biotinylated Recombinant ICOS Fc Chimera (Catalog # BT169) binds with an ED50 of 1-6 ng/mL.

Formulation, Preparation, and Storage

BT169
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution

Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ICOS

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References

  1. Aicher, A. et al. (2000) J. Immunol. 164:4689.
  2. Coyle, A.J. et al. (2000) Immunity 13:95.
  3. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  5. Hutloff, A. et al. (1999) Nature 397:263.
  6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

Inducible Co-stimulator

Alternate Names

AILIM, CD278, CRP-1

Entrez Gene IDs

29851 (Human); 54167 (Mouse); 102116512 (Cynomolgus Monkey)

Gene Symbol

ICOS

UniProt

Q9Y6W8

Additional ICOS Products

Product Documents for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

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Product Specific Notices for Recombinant Human ICOS Fc Chimera Biotinylated Protein, CF

For research use only

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