Recombinant Human IFN-alpha/beta R2 Fc Chimera Protein, CF

(1 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit Type-I IFN-mediated anti-viral activity. The ED50 for this effect, as measured by inhibition of Recombinant Human IFN‑ beta (Catalog # 8499-IF) , is typically 0.1-0.6 μg/mL
  • Source
    Mouse myeloma cell line, NS0-derived
    Human IFN-alpha / beta R2
    Accession # P48551
    IEGRMD Human IgG1
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    51.3 (monomer) kDa
    71-76 kDa, reducing conditions
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human IFN-alpha/beta R2 Fc Chimera (Catalog #4015-AB) inhibits the anti-viral activity ofRecombinant Human IFN-beta (Catalog # 8499-IF). The ED50 for thiseffect is typically 0.1-0.6 μg/mL.
Background: IFN-alpha/beta R2

IFN-alpha / beta R2, also known as IFNAR2, is a 100 kDa glycoprotein in the class II cytokine receptor family. These proteins form heterodimeric receptor complexes that transduce signals from the interferon, IL-10, and IL-28 families of cytokines (1, 2). IFN-alpha / beta R2, in association with IFN-alpha / beta R1, is required for mediating the antiviral, antiproliferative, and apoptotic effects of the type I interferons IFN-alpha and IFN-beta. IFN-alpha / beta R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-alpha / beta R1, resulting in the formation of a signaling ternary receptor complex (3, 4). Mature human IFN-alpha / beta R2 consists of a 217 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, a 21 aa transmembrane segment, and a 251 aa cytoplasmic domain. Alternate splicing generates a secreted isoform that corresponds to the ECD and a 50 kDa transmembrane isoform with a substituted and truncated cytoplasmic region (5, 6). The short isoform is impaired in its ability to activate signaling molecules and functions as a dominant negative receptor subunit (7 - 9). IFN-alpha / beta R2 is also subject to presenilin-dependent intramembrane proteolysis, resulting in the liberation of nearly the entire ECD as well as the cytoplasmic domain which migrates to the nucleus and can inhibit gene transcription (10). High concentrations of soluble IFN-alpha / beta R2 bind and neutralize IFN-alpha and IFN-beta, while lower concentrations prolong the antiviral activity of circulating IFN-beta but not IFN-alpha (11). Human but not mouse IFN-alpha / beta R2 constitutively associates with STAT4, which may account for species specific differences observed in type I interferon responses (12). Within the ECD, human IFN-alpha / beta R2 shares 63%, 60%, and 48% aa sequence identity with bovine, mouse, and ovine IFN-alpha / beta R2, respectively.

  • References:
    1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
    2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
    3. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
    4. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
    5. Lutfalla, G. et al. (1995) EMBO J. 14:5100.
    6. Novick, D. et al. (1995) J. Leukocyte Biol. 57:712.
    7. Pfeffer, L.M. et al. (1997) J. Biol. Chem. 272:11002.
    8. Gazziola, C. et al. (2005) Int. J. Oncol. 26:129.
    9. Kotenko, S.V. and S. Pestka (2000) Oncogene 19:2557.
    10. Saleh, A.Z.M. et al. (2004) Oncogene 23:7076.
    11. McKenna, S.D. et al. (2004) J. Interferon Cytokine Res. 24:119.
    12. Tyler, D.R. et al. (2007) Mol. Immunol. 44:1864.
  • Long Name:
    Interferon alpha/beta Receptor 2
  • Entrez Gene IDs:
    3455 (Human); 15976 (Mouse)
  • Alternate Names:
    beta receptor10; human interferon alpha; human interferon alpha/beta receptor; IFNABR; IFN-alpha binding protein; IFN-alpha/beta receptor 2; IFN-alpha-REC; IFNAR2; IFN-aR2; IFNARB; IFNbR2; IFN-bR2; IFN-R; IFN-R-2; interferon (alpha, beta and omega) receptor 2; Interferon alpha binding protein; interferon alpha/beta receptor 2; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Novaferon, a novel recombinant protein produced by DNA-shuffling of IFN-alpha, shows antitumor effect in vitro and in vivo.
    Authors: Li M, Rao C, Pei D, Wang L, Li Y, Gao K, Wang M, Wang J
    Cancer Cell Int, 2014;14(1):8.
    Species: N/A
    Sample Type: CM5 chip
    Application: Surface Plasmon Resonance
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Recombinant Proteins
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Recombinant Human IgG1 Fc, CF

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