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Recombinant Human IGF-I/IGF-1 GMP Protein, CF

Name: Recombinant Human IGF-I/IGF-1 GMP Protein, CF
Brand: R&D Systems
SKU: 291-GMP
Price: 876 USD
Availability: InStock

R&D Systems | Catalog # 291-GMP

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Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Manufacturing Specifications
Specific Notices
Research Areas

Key Product Details

A DMF is on file for this protein

Source

E. coli

Accession Number

P05019.1

Applications

Bioactivity

View all IGF-I/IGF-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IGF-I/IGF-1 protein
Gly49-Ala118
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly-Pro-Glu-Thr-Leu-(Cys)-Gly-Ala-Glu-Leu

Predicted Molecular Mass

7.6 kDa

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED₅₀ for this effect is 0.3-1.5 ng/mL.

The specific activity of recombinant human IGF-I/IGF-1 is >500 IU/mg, which is calibrated against the human IGF-I/IGF-1 WHO International Standard (NIBSC code: 91/554).

Host Cell Protein

<1.0 ng per µg of protein when tested by ELISA.

Mycoplasma

Negative for Mycoplasma.

Host Cell DNA

<0.0015 ng per µg of protein when tested by PCR.

Scientific Data Images for Recombinant Human IGF-I/IGF-1 GMP Protein, CF

Recombinant Human IGF-I/IGF-1 GMP Protein Bioactivity

GMP-grade Recombinant Human IGF-I/IGF-1 (Catalog # 291-GMP) stimulates cell proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner. The ED₅₀ for this effect is 0.3‑1.5 ng/mL.

Recombinant Human IGF-I/IGF-1 GMP Protein SDS-PAGE

1 μg/lane of Recombinant Human GMP-grade IGF-I/IGF-1 (Catalog # 291-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

Recombinant Human IGF-I/IGF-1 GMP Protein Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human IGF-I/IGF-1 (Catalog # 291-GMP). The major peak at 7648 Da corresponds to the measured molecular weight of the intact protein. The calculated mass is 7655 Da.

Formulation, Preparation, and Storage

291-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IGF-I/IGF-1

Insulin-like growth factor 1 (IGF-1 or IGF-I), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C‑terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF‑1 is identical between isoforms and is generated by proteolytic removal of the N- and C‑terminal regions. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF2. Circulating IGF-1 is produced by hepatocytes, while local IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4‑7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-1 binds IGF1R, IGF2R, and the insulin receptor, although its effects are mediated primarily by IGF1R (9). IGF-1 association with IGF binding proteins increases its plasma half‑life and modulates its interactions with receptors (10).

References

Philippou, A. et al. (2007) In Vivo 21:45.
Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

P05019.1

Additional IGF-I/IGF-1 Products

Product Documents for Recombinant Human IGF-I/IGF-1 GMP Protein, CF

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented and controlled manufacturing process
Control of documentation and process changes by QA
Personnel training programs
Raw material inspection and vendor qualification/monitoring program
Validated equipment, processes and test methods
Equipment calibration and maintenance schedules using a Regulatory Asset Manager
Facility/Utilities maintenance, contamination controls, safety and pest control programs
Material review process for variances
Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

N-terminal amino acid analysis
SDS-PAGE purity analysis
Molecular weight analysis via mass spectrometry
Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
Bioassay analysis
Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
Host cell protein assessment
Host cell DNA assessment
Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production