Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1241-S8-050
Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF Binding Activity
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Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IGSF8/CD316 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant CD81 Fc Chimera binds with an ED50 of 1.2-7.2 μg/mL
Source
Human embryonic kidney cell, HEK293-derived human IGSF8/CD316 protein
Human IGSF8
(Arg28-Thr579)
Accession # Q969P0-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Arg28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85 kDa
SDS-PAGE
86-113 kDa, reducing conditions

Product Datasheets

Data Images

Binding Activity Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF Binding Activity View Larger

When Recombinant Human IGSF8/CD316 Fc Chimera (Catalog# 1241-S8) is immobilized at 1 µg/mL (100 µL/well), Biotinylated RecombinantCD81 Fc Chimera binds with an ED50 of 1.2-7.2 μg/mL.

SDS-PAGE Recombinant Human IGSF8/CD316 Fc Chimera Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human IGSF8/CD316 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 86-113 kDa and 170-230 kDa, respectively.

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Background: IGSF8/CD316

IGSF8 (Immunoglobulin superfamily member 8), also known as EWI-2, KCT-4, LIR-D1, and PGRL, is a 75-kDa cell surface protein belonging to the immunoglobulin superfamily (1). IGSF8 is widely expressed, with pronounced mRNA expression in the brain and protein expression on peripheral blood lymphocytes and hepatocytes where it colocalizes with CD81 (1-3). It strongly associates with tetraspanins CD9 and CD81 which may act as physical linkers to form a complex with alpha 3 beta 1 integrin that may regulate cell aggregation and motility on laminin-5 (4). Human IGSF8 is synthesized as a 613 aa protein that includes a 27 aa signal peptide, a 552 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic tail. Within the ECD, human IGSF8 shares 91% and 90% aa sequence identity with mouse and rat IGSF8, respectively. IGSF8 is an inducible receptor for Heat Shock Protein A8 (HSPA8) on activated dendritic cells (5). IGSF8 can interact with alpha -Actinin to regulate T cell immune synapses and HIV viral infection (6). In human glioma patients, low IGSF8 expression correlates with shorter survival time. Studies have shown that re-expression of IGSF8 in malignant glioblastoma cell lines inhibited glioblastoma colony formation in soft agar and caused diminished cell motility and invasion (7).

References
  1. Clark, K.L. et al. (2001) J. Immunol. 167:5115.
  2. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
  3. Charrin, S. et al. (2003) Biochem. J. 373:409.
  4. Stipp, C.S. et al. (2003) J. Cell Biol. 163:1167.
  5. Kettner, S. et al. (2007) Mol Cell Biol. 27:7718.
  6. Gordón-Alonso, M. et al. (2012) J Immunol 189:689.
  7. Kolesnikova, T. et al. (2009) Neoplasia.11:77.
Long Name
Immunoglobulin Superfamily, Member 8
Entrez Gene IDs
93185 (Human); 140559 (Mouse)
Alternate Names
CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IGSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8

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