Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

Name: Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF
Brand: R&D Systems
SKU: AVI10930
Price: 592 USD
Availability: InStock

R&D Systems | Catalog # AVI10930

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Biotinylated

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein (AVI10930)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q99665.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-12 R beta 2 Proteins and Enzymes »

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 2 protein

Human IL-12 RB2 (Cys28-Asn622) Accession # Q99665.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys28

Predicted Molecular Mass

96 kDa

SDS-PAGE

115-130 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-12 Protein (Catalog # 219-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL‑12 R beta 2 Fc Chimera Avi-tag (Catalog# AVI10930) that produces 50% of the optimal binding response is 0.15‑1.50 μg/mL.

Scientific Data Images for Recombinant Human IL-12 R beta 2 Fc Avi-tag Protein, CF

When Recombinant Human IL-12 Protein (Catalog# 219-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL‑12R beta 2 Fc Chimera Avi-tag (Catalog # AVI10930) that produces 50% of the optimal binding response is 0.15‑1.50 μg/mL.

Biotinylated Recombinant Human IL‑12 R beta 2 Fc Chimera Avi-tag Protein SDS-PAGE.

1 μg/lane of Biotinylated Recombinant Human IL‑12 R beta 2 Fc Chimera Avi-tag Protein (Catalog # AVI10930) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized bysilver staining, showing bands at 115-130 kDa and 230-260 kDa, respectively.

Formulation, Preparation, and Storage

AVI10930

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-12 R beta 2

IL-12 Receptor beta 2 (IL-12 R beta 2) is a member of the cytokine receptor superfamily and when combined with the highly homologous IL-12 R beta 1, forms the heterodimeric high affinity IL-12 receptor complex. Both receptors are type I transmembrane glycoproteins with homology to gp130, the common receptor beta -chain of the IL-6-like cytokine superfamily (ref). Mature human IL-12 R beta 2 consists of an extracellular domain (ECD) with five fibronectin type III (FnIII) domains and a WSXWS motif, a single transmembrane domain and a cytoplasmic region with a Box 1 motif and a tyrosine phosphorylation site that both mediate intracellular signaling (1). The ECD of human IL-12 R beta 2 shares 69% and 67% amino acid sequence identity with mouse and rat IL-12 R beta 2, respectively. Human and mouse IL-12 R beta 2 do not bind cross-species IL-12 (2). Alternative splicing creates a IL-12 R beta 2 with a shortened and altered cytoplasmic sequence (3). IL-12 R beta 1 and IL-12 R beta 2 are receptors for the IL-12 family, which includes IL-12, IL-23, IL-27 and IL-35 and they activate the Janus kinase (JAK)–STAT (signal transducer and activator of transcription) pathway of signal transduction (4). The receptor chains are utilized by the cytokines in different combinations, with IL-12RB2 binding to IL-12 and IL-35 (5). Unlike IL-12RB1, which is constitutive in T cells, NK cells and B cells, IL-12 R beta 2 expression is more limited (2). IL-12 R beta 2 is expressed following STAT1 activation by IFN-gamma, IL-27 and/or T cell receptor stimulation of naïve T cells, allowing IL-12 to promote Th1, but not Th2, differentiation (6-8). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of mouse IL-12 R beta 2 causes systemic overexpression of IL-6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 9-11). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies (2, 9-11). In humans, polymorphism of the IL-12 R beta 2 gene is associated with systemic sclerosis (12). Our Avi-tag Biotinylated IL-12 R beta 2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.