Recombinant Human IL-12 R beta 2 Fc Chimera Protein, CF

Name: Recombinant Human IL-12 R beta 2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1959-B2

Catalog #: 1959-B2 Datasheet / COA / SDS

Newer Version Available: 1959-B2B

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Discontinued Product

1959-B2 has been discontinued and is replaced by 1959-B2B.

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All IL-12 R beta 2 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-12 R beta 2 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized rhIL-12 R beta 2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhIL-12 with a linear range of 0.8-50 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human IL-12 R beta 2 protein

Human IL-12 R beta 2 (Cys28-Asn622) Accession # Q99665	IEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Accession #

Q99665

N-terminal Sequence
Analysis

Cys28

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

94.6 kDa (monomer)

SDS-PAGE

125 kDa, reducing conditions

Product Datasheets

1959-B2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1959-B2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-12 R beta 2

The high-affinity IL-12 receptor complex includes the 100 kDa IL-12 receptor beta 1 (IL-12 R beta 1) and the 130 kDa IL-12 Receptor beta 2 (IL-12 R beta 2) subunits, both type I transmembrane proteins within the cytokine receptor superfamily (1, 2). Its ligand, IL-12, is a disulfide-linked dimer of 35 kDa (IL-12 alpha p35) and 40 kDa (IL-12 beta p40) subunits. IL-12 R beta 2 binds IL-12 alpha and signals through Jak2, while IL-12 R beta 1 binds IL-12 beta and signals through Tyk2 (3). IL-12 R beta 1 is also a subunit of the IL-23 receptor complex (3). The 874 amino acid (aa) human IL-12 R beta 2 includes a 23 aa signal peptide, a 599 aa extracellular domain (ECD) with five fibronectin type III (Fn III) domains, 8 potential N-glycosylation sites, and a WSXWS motif, a 21 aa transmembrane domain and a 219 aa cytoplasmic region with a Box 1 motif and a tyrosine phosphorylation site that both mediate intracellular signaling (3). Human IL-12 R beta 2 ECD shares 69%, 67%, 79%, 81% and 82% aa sequence identity with mouse, rat, canine, porcine and bovine IL-12 R beta 2, respectively. Human and mouse IL-12 R beta 2 do not bind cross-species IL-12 (2). A human alternatively spliced 659 aa form contains a shortened, altered cytoplasmic sequence (4). Unlike IL-12 R beta 1, which is constitutive in T cells, NK cells and B cells, IL-12 R beta 2 expression is more limited (2). IL-12 R beta 2 is expressed following STAT1 activation by IFN-gamma, IL-27 and/or T cell receptor stimulation of naïve T cells, allowing IL-12 to promote Th1, but not Th2, differentiation (5-7). Among B cells, surface expression is limited to naïve germinal center and memory B cells, and myeloma cells (2). Deletion of mouse IL-12 R beta 2 causes systemic overexpression of IL-6, accelerated maturation of thymocytes, deficient regulatory T cell maturation and function, and reduced splenic T cell apoptosis (2, 8-10). These mice are susceptible to autoimmune diseases such as experimental autoimmune encephalitis and spontaneous B cell malignancies
(2, 8-10). In humans, polymorphism of the IL-12 R beta 2 gene is associated with systemic sclerosis (11).

References

Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:14002.
Pistoia, V. et al. (2009) J. Clin. Oncol. 27:4809.
Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
SwissProt accession # Q99665
Rogge, L. et al. (1997) J. Exp. Med. 185:825.
Becskei, A. and M.J. Grusby (2007) FEBS Lett. 581:5199.
Szabo, S.J. et al. (1997) J. Exp. Med. 185:817.
Zhao, Z. et al. (2008) J. Immunol. 181:3870.
Gran, B. et al. (2010) Exp. Mol. Pathol. 89:126.
Airoldi, I. et al. (2005) Blood 106:3846.
Bossini-Castillo, L. et al. (2011) Hum. Mol. Genet. Nov. 29 [Epub ahead of print]

Long Name

Interleukin 12 Receptor beta 2

Entrez Gene IDs

3595 (Human); 16162 (Mouse)

Alternate Names

IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2