hh Recombinant Human IL-5 Protein 205-IL-005: R&D Systems

Recombinant Human IL-5 Protein

(26 citations)   
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.04-0.2 ng/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (stably transfected)-derived Ile20-Ser134
  • Accession #
  • N-terminal Sequence
    Analysis
    Ile20
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    13 kDa (monomer)
  • SDS-PAGE
    13-15 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
205-IL
 
205-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Data Images
Recombinant Human IL-5 (Catalog # 205-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.04-0.2 ng/mL.
1 μg/lane of Recombinant Human IL-5 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 13-15 kDa and 28-32 kDa, respectively.
Background: IL-5

Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1 ‑ 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively and shows cross‑reactivity with mouse IL-5. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1 ‑ 3, 6 ‑ 8). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 9, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

  • References:
    1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
    2. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
    3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
    4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
    5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
    6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
    7. Lalani, T. et al. (1999) Ann. Allergy Asthma Immunol. 82:317.
    8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
    9. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
    10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
    11. Tavernier, J. et al. (1991) Cell 66:1175.
    12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
    13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
    14. Tavernier, J. et al. (2000) Blood 95:1600.
    15. Kopf, M. et al. (1996) Immunity 4:15.
    16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
    17. Denburg, J. A. et al. (1991) Blood 77:1462.
  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen
    Authors: JT Schroeder, AP Bieneman
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Human eosinophils constitutively express a unique serine protease, PRSS33
    Authors: S Toyama, N Okada, A Matsuda, H Morita, H Saito, T Fujisawa, S Nakae, H Karasuyama, K Matsumoto
    Allergol Int, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. Functional IL-33 receptors are expressed in early progenitor stages of allergy-related granulocytes
    Immunology, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. The effect of hepatocyte growth factor on secretory functions in human eosinophils
    Cytokine, 2016;88(0):45-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. SOCS3 silencing attenuates eosinophil functions in asthma patients.
    Authors: Zafra M, Canas J, Mazzeo C, Gamez C, Sanz V, Fernandez-Nieto M, Quirce S, Barranco P, Ruiz-Hornillos J, Sastre J, del Pozo V
    Int J Mol Sci, 2015;16(3):5434-51.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24).
    Authors: Dyer, Kimberly, Rosenberg, Helene F
    PLoS ONE, 2015;10(3):e0118887.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. IL-25 induces airways angiogenesis and expression of multiple angiogenic factors in a murine asthma model.
    Authors: Yao, Xiujuan, Wang, Wei, Li, Yan, Huang, Ping, Zhang, Qian, Wang, Jingjing, Wang, Wen, Lv, Zhe, An, Yunqing, Qin, Jianguo, Corrigan, Chris J, Huang, Kewu, Sun, Yongchan, Ying, Sun
    Respir Res, 2015;16(0):39.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Eosinophil-specific deletion of IkappaBalpha in mice reveals a critical role of NF-kappaB-induced Bcl-xL for inhibition of apoptosis.
    Authors: Schwartz C, Willebrand R, Huber S, Rupec R, Wu D, Locksley R, Voehringer D
    Blood, 2015;125(25):3896-904.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Functional analysis of free fatty acid receptor GPR120 in human eosinophils: implications in metabolic homeostasis.
    Authors: Konno, Yasunori, Ueki, Shigehar, Takeda, Masahide, Kobayashi, Yoshiki, Tamaki, Mami, Moritoki, Yuki, Oyamada, Hajime, Itoga, Masamich, Kayaba, Hiroyuki, Omokawa, Ayumi, Hirokawa, Makoto
    PLoS ONE, 2015;10(3):e0120386.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Cholesterol selectively regulates IL-5 induced mitogen activated protein kinase signaling in human eosinophils.
    Authors: Burnham M, Esnault S, Roti Roti E, Bates M, Bertics P, Denlinger L
    PLoS ONE, 2014;9(8):e103122.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  12. Cytokines alter IgA1 O-glycosylation by dysregulating C1GalT1 and ST6GalNAc-II enzymes.
    Authors: Suzuki, Hitoshi, Raska, Milan, Yamada, Koshi, Moldoveanu, Zina, Julian, Bruce A, Wyatt, Robert J, Tomino, Yasuhiko, Gharavi, Ali G, Novak, Jan
    J Biol Chem, 2014;289(8):5330-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  13. Natural killer cells induce eosinophil activation and apoptosis.
    Authors: Awad A, Yassine H, Barrier M, Vorng H, Marquillies P, Tsicopoulos A, Duez C
    PLoS ONE, 2014;9(4):e94492.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Tumour necrosis factor-alpha regulates human eosinophil apoptosis via ligation of TNF-receptor 1 and balance between NF-kappaB and AP-1.
    Authors: Kankaanranta H, Ilmarinen P, Zhang X, Adcock I, Lahti A, Barnes P, Giembycz M, Lindsay M, Moilanen E
    PLoS ONE, 2014;9(2):e90298.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  15. Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils.
    Authors: Oh, Jiyoung, Malter, James S
    J Immunol, 2013;190(10):4937-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  16. Osteopontin is expressed and functional in human eosinophils.
    Authors: Puxeddu I, Berkman N, Ribatti D
    Allergy, 2010;65(2):168-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  18. Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
    Authors: Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB
    Am. J. Respir. Cell Mol. Biol., 2006;35(4):503-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  19. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  20. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
    Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
    Eur. J. Immunol., 2006;36(7):1975-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  21. Pharmacological characterization of guinea pig chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2).
    Authors: Liu F, Gonzalo JA, Manning S, O&amp;amp;apos;Connell LE, Fedyk ER, Burke KE, Elder AM, Pulido JC, Cao W, Tayber O, Qiu Y, Ghosh S, Ocain TD, Hodge MR, Suzuki-Yagawa Y
    Prostaglandins Other Lipid Mediat., 2005;76(1):133-47.
    Species: Guinea Pig
    Sample Type: Whole Cells
    Application: Bioassay
  22. Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
    Authors: Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
    Invest. Ophthalmol. Vis. Sci., 2005;46(3):797-802.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  23. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways.
    Authors: Kao CY, Chen Y, Thai P, Wachi S, Huang F, Kim C, Harper RW, Wu R
    J. Immunol., 2004;173(5):3482-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  24. Eosinophils adhere to vascular cell adhesion molecule-1 via podosomes.
    Authors: Johansson MW, Lye MH, Barthel SR, Duffy AK, Annis DS, Mosher DF
    Am. J. Respir. Cell Mol. Biol., 2004;31(4):413-22.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  25. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects.
    Authors: Hellman C, Hallden G, Hylander B, Lundahl J
    Clin. Exp. Immunol., 2003;131(1):75-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  26. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
    Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
    Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 26 Citations

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