Recombinant Human IL-9 Protein, CF

R&D Systems | Catalog # 11081-IL

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human IL-9 Protein (11081-IL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

P15248.1

Applications

Bioactivity
View all IL-9 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-9 protein
Gln19-Ile144

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln19 inferred from enzymatic pyroglutamate treatment revealing Gly20

Predicted Molecular Mass

14 kDa

SDS-PAGE

26-38 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 0.200-1.00 ng/mL

Scientific Data Images for Recombinant Human IL-9 Protein, CF

Recombinant Human IL‑9 Protein Bioactivity.

Recombinant Human IL­-9  (Catalog # 11081-IL) induces  MO7e human megakaryocytic leukemic cell proliferation. The  ED50 for this effect is 0.200‑1.00 ng/mL

Recombinant Human IL‑9 Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑9 Protein (Catalog # 11081-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26-38 kDa.

Formulation, Preparation, and Storage

11081-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9 (14, 15).

References

  1. Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
  2. Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
  3. Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
  4. Townsend, M.J. et al. (2000) Immunity 13:573.
  5. Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
  6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
  7. Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
  8. Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
  9. Tan, C. et al. (2010) J. Immunol. 185:6795.
  10. Jager, A. et al. (2009) J. Immunol. 183:7169.
  11. Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
  12. Lu, L.-F. et al. (2006) Nature 442:997.
  13. Stassen, M. et al. (2000) J. Immunol. 164:5549.
  14. Yang, Y.-C. et al. (1989) Blood 74:1880.
  15. Renauld, J.-C. et al. (1990) Cytokine 2:9.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

UniProt

P15248.1

Additional IL-9 Products

Product Documents for Recombinant Human IL-9 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-9 Protein, CF

For research use only

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